Strain screening and sodium lactate effect on spiramycin production in Streptomyces spiramyceticus

详细信息    查看全文

• 作者：Jun Zeng ; Rui-Fang Ye ; Yi-Tao Zheng ; Quan-Gui Mao…
• 关键词：Streptomyces spiramyceticus ; Spiramycin ; Sodium lactate ; Sodium lactate tolerant mutant ; Fermentation
• 刊名：Research on Chemical Intermediates
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：42
• 期：3
• 页码：1627-1638
• 全文大小：1,004 KB
• 参考文献：1.S. Omura, H. Takeshima, A. Nakagawa, J. Miyazawa, G. Lukacs, Biochemistry 16, 2860 (1977)CrossRef
  2.S. Omura, C. Kitao, H. Hamada, H. Ikeda, Chem. Pharm. Bull. 27, 176 (1979)CrossRef
  3.J. Neusil, Z. Hostalek, Folia Microbiol. 31, 402 (1986)CrossRef
  4.S. Khaoua, A. Lebrihi, M. Laakel, F. Schneider, P. Germain, G. Lefebvre, Appl. Microbiol. Biotechnol. 36, 763 (1992)
  5.C.C. Hua, L.Z. Hui, Z. Lei, C. Ying, Chin. J. Antibiot. 20, 392 (1995)
  6.J.Z. Hua, S.Y. Li, H.C. Nong, Chin. J. Antibiot. 17, 265 (1992)
  7.J.Z. Hua, Chin. J. Biotechnol. 11, 295 (1995)
  8.A. Lounes, A. Lebrihi, C. Benslimane, G. Lefebvre, P. Germain, Curr. Microbiol. 31, 304 (1995)CrossRef
  9.A. Lounes, A. Lebrihi, C. Benslimane, G. Lefebvre, P. Germain, Process Biochem. 31, 13 (1996)CrossRef
  10.V. Colombie, C. Bideaux, G. Goma, J.L. Uribelarrea, Bioprocess Biosyst. Eng. 28, 55 (2005)CrossRef
  11.A. Lebrihi, D. Lamsaif, G. Lefebvre, P. Germain, Appl. Microbiol. Biotechnol. 37, 382 (1992)CrossRef
  12.A. Lounes, A. Lebrihi, C. Benslimane, G. Lefebvre, P. Germain, Can. J. Microbiol. 41, 800 (1995)CrossRef
  13.M. Laakel, A. Lebrihi, S. Khaoua, F. Schneider, G. Lefebvre, P. Germain, Microbiobgy 140, 1451 (1994)CrossRef
  14.S. Untrau, A. Lebrihi, G. Lefebvre, P. Germain, Curr. Microbiol. 28, 111 (1994)CrossRef
  15.S. Untrau, A. Lebrihi, P. Germain, G. Lefebvre, Curr. Microbiol. 25, 313 (1992)CrossRef
  16.M. Laakel, A. Lebrihi, S. Khaoua, F. Schneider, G. Lefebvre, P. Germain, Can. J. Microbiol. 40, 672 (1994)CrossRef
  17.A. Lounes, A. Lebrihi, C. Benslimane, G. Lefebvre, P. Germain, Appl. Microbiol. Biotechnol. 45, 204 (1996)CrossRef
  18.S.U. Taghian, A. Lebrihi, P. Germain, G. Lefebvre, Can. J. Microbiol. 41, 157 (1995)CrossRef
  19.M.A. Richardson, S. Kuhstoss, M.L.B. Huber, L. Ford, O. Godfrey, J.R. Turner, R.N. Rao, J. Bacteriol. 172, 3790 (1990)
  20.F. Karray, E. Darbon, H.C. Nguyen, J. Gagnat, J.L. Pernodet, J. Bacteriol. 192, 5813 (2010)CrossRef
  21.H.C. Nguyen, F. Karray, S. Lautru, J. Gagnat, A. Lebrihi, T.D.H. Huynh, J.L. Pernodet, Antimicrob. Agents Chemother. 54, 2830 (2010)CrossRef
  22.H.C. Nguyen, E. Darbon, R. Thai, J.L. Pernodet, S. Lautru, Antimicrob. Agents Chemother. 57, 3836 (2013)CrossRef
  23.C.J. Hong, X.L. Di, Antibiotic Industrial Analysis (China Medical Science Press, Beijing, 1991), p. 126
  24.L.Y. Yuan, C.C. Hua, T. Ping, Chin. J. Chromatogr. 20, 46 (2002)
  25.O.H. Lowry, N.J. Rosebrough, A.L. Farr, R.J. Randall, J. Biol. Chem. 193, 265 (1951)
  26.W.J. Yan, Z.S. Geng, X.C. Fa, Biochemistry (Higher Education Press, Beijing, 2008), p. 158
  
• 作者单位：Jun Zeng (1)
  Rui-Fang Ye (1)
  Yi-Tao Zheng (1)
  Quan-Gui Mao (1)
  He-Ping Lv (1)
  Ting-Ting Shi (1)
  
  1. The State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Catalysis
  Physical Chemistry
  Inorganic Chemistry
  
• 出版者：Springer Netherlands
• ISSN：1568-5675

文摘

Strain improvement and addition of sodium lactate to fermentation medium to enhance the productivity of spiramycin were performed. Of the sodium lactate tolerant mutants that were screened, one mutant, Streptomyces spiramyceticus 16-10-12, produced 23 % more spiramycin than the original strain, Streptomyces spiramyceticus 5-1. The effect of sodium lactate on spiramycin production with S. spiramyceticus 16-10-12 was studied. The titer was improved by 16.9 % with the addition of 15 g L−1 sodium lactate in the fermentation medium at the beginning. The results from using the new process in a 15 L bioreactor showed that there were more precursors in fermentation broth with a sodium lactate tolerant mutant, and that these precursors were used more than with the original strain. After adding sodium lactate, the titer was increased by 23.4 %, because the flux to TCA circulation was increased, more precursors had been produced and the activities of Acyl-CoA synthetases, Acylphosphotransferases and Acylkinases in synthesis phase were also increased.