Regulation of cellulase expression, sporulation, and morphogenesis by velvet family proteins in Trichoderma reesei
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- 作者：Kuimei Liu ; Yanmei Dong ; Fangzhong Wang…
- 关键词：Velvet family ; Sporulation ; Cellulase regulation ; Morphogenesis ; Trichoderma reesei
- 刊名：Applied Microbiology and Biotechnology
- 出版年：2016
- 出版时间：January 2016
- 年：2016
- 卷：100
- 期：2
- 页码：769-779
- 全文大小：1,950 KB
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Yanmei Dong (1)
Fangzhong Wang (1)
Baojie Jiang (1)
Mingyu Wang (1)
Xu Fang (1)
1. State Key Laboratory of Microbial Technology, School of Life Sciences, Shandong University, Jinan, 250100, China - 刊物类别：Chemistry and Materials Science
- 刊物主题：Chemistry
Biotechnology
Microbiology
Microbial Genetics and Genomics - 出版者：Springer Berlin / Heidelberg
- ISSN：1432-0614
文摘
Homologs of the velvet protein family are encoded by the ve1, vel2, and vel3 genes in Trichoderma reesei. To test their regulatory functions, the velvet protein-coding genes were disrupted, generating Δve1, Δvel2, and Δvel3 strains. The phenotypic features of these strains were examined to identify their functions in morphogenesis, sporulation, and cellulase expression. The three velvet-deficient strains produced more hyphal branches, indicating that velvet family proteins participate in the morphogenesis in T. reesei. Deletion of ve1 and vel3 did not affect biomass accumulation, while deletion of vel2 led to a significantly hampered growth when cellulose was used as the sole carbon source in the medium. The deletion of either ve1 or vel2 led to the sharp decrease of sporulation as well as a global downregulation of cellulase-coding genes. In contrast, although the expression of cellulase-coding genes of the ∆vel3 strain was downregulated in the dark, their expression in light condition was unaffected. Sporulation was hampered in the ∆vel3 strain. These results suggest that Ve1 and Vel2 play major roles, whereas Vel3 plays a minor role in sporulation, morphogenesis, and cellulase expression. Keywords Velvet family Sporulation Cellulase regulation Morphogenesis Trichoderma reesei