Effect of reversine on cell cycle, apoptosis, and activation of hepatic stellate cells
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- 作者：Yu Huang ; Di Huang ; Jiefeng Weng ; Shuai Zhang…
- 关键词：Reversine ; Hepatic stellate cell ; Cell cycle ; Apoptosis ; Extracellular matrix
- 刊名：Molecular and Cellular Biochemistry
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：423
- 期：1-2
- 页码：9-20
- 全文大小：8,923 KB
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Life Sciences
Biochemistry
Medical Biochemistry
Oncology
Cardiology - 出版者：Springer Netherlands
- ISSN：1573-4919
- 卷排序：423
文摘
Experimental and clinical evidence show that liver fibrosis is potentially reversible. Hepatic stellate cells (HSCs) play a key role in the development of liver fibrosis. Some studies have shown that reversine could induce cell apoptosis. We attempted to elucidate the effect of reversine on cell cycle, apoptosis, and activation of HSCs. Data showed that reversine induced morphological changes in HSCs, inhibited cell proliferation, and induced cell-cycle arrest at the G2/M phase. Reversine induced cell apoptosis through caspase-dependent and mitochondria-dependent pathways. Reversine inhibited the activation of HSCs through TGF-β signaling pathway and degraded extracellular matrix protein collagen-I. The decreased TIMP1 and TGF-β1 proteins promoted fibrosis reversion. Reversine might be a promising drug for liver fibrosis reversion because it induces HSCs apoptosis, restrains cell proliferation, reduces HSCs activation, and degrades extracellular matrix in vitro.