A reappraisal of Aspergillus section Nidulantes with descriptions of two new sterigmatocystin-producing species

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• 作者：Vit Hubka ; Alena Nováková ; Stephen W. Peterson…
• 关键词：Aspergillus nidulans ; Codon usage ; Emericella ; Mycotoxins ; Paralogous genes ; Subgeneric classification of Aspergillus
• 刊名：Plant Systematics and Evolution
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：302
• 期：9
• 页码：1267-1299
• 全文大小：4,386 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  Plant Ecology
  Plant Anatomy and Development
  Plant Systematics/Taxonomy/ Biogeography
  
• 出版者：Springer Wien
• ISSN：1615-6110
• 卷排序：302

文摘

Aspergillus section Nidulantes is a speciose group of microscopic fungi whose species are important in indoor air quality, food spoilage, mycotoxin production and human pathogenicity. We assembled as many species from the section as possible with either type specimens or protologues for analysis. DNA sequences were obtained from up to four loci to conduct phylogenetic analysis of sect. Nidulantes and other species in subgenus Nidulantes. Comparisons of phenetic characters were made. Our goal was to recognize monophyletic taxa for section Nidulantes as well as the other taxa in the subgenus. Phylogenetic analysis indicated that sections Versicolores and Aenei formed subclades in the larger clade containing the type species of section Nidulantes. In order to maintain monophyly in section Nidulantes, we synonymized sects. Versicolores and Aenei with sect. Nidulantes. In place of sections, we have identified six subclades in sect. Nidulantes that also have shared phenetic character sets that allow for easier morphological identification of species. Earlier reports showed that standard β-tubulin primers can occasionally amplify the tubC gene rather than the benA gene they were designed for. We show that codon usage analysis can easily distinguish between these paralogues, and we provide primer pair options to allow benA amplifications. Two new sect. Nidulantes species are described from European caves, A. croceus (Spain) and A. askiburgiensis (Czech Republic). The analysis of exometabolites showed that both species are able to produce the mycotoxin sterigmatocystin and some additional compounds.