Nucleic acids digestion by enzymes in the stomach of snakehead (Channa argus) and banded grouper (Epinephelus awoara)

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• 作者：Yu Liu ; Yanfang Zhang ; Wei Jiang ; Jing Wang…
• 关键词：Fish ; External nucleic acids ; Feed ; Pepsin ; Stomach
• 刊名：Fish Physiology and Biochemistry
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：43
• 期：1
• 页码：127-136
• 全文大小：
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Freshwater & Marine Ecology; Animal Physiology; Animal Anatomy / Morphology / Histology; Animal Biochemistry; Zoology;
• 出版者：Springer Netherlands
• ISSN：1573-5168
• 卷排序：43

文摘

Dietary nucleic acids (NAs) were important nutrients. However, the digestion of NAs in stomach has not been studied. In this study, the digestion of NAs by enzymes from fish stomach was investigated. The snakehead pepsins (SP) which were the main enzymes in stomach were extracted and purified. The purity of SP was evaluated by SDS-PAGE and HPLC. The snakehead pepsin 2 (SP2) which was the main component in the extracts was used for investigating the protein and NAs digestion activity. SP2 could digest NAs, including λ DNA and salmon sperm DNA. Interestingly, the digestion could be inhibited by treatment of alkaline solution at pH 8.0 and pepstatin A, and the digestion could happen either in the presence or absence of hemoglobin (Hb) and BSA as the protein substrates. Similarly, the stomach enzymes of banded grouper also showed the NAs digestion activity. NAs could be digested by the stomach enzymes of snakehead and banded grouper. It may be helpful for understanding both animal nutrition and NAs metabolic pathway.