Validation of simple sequence repeats associated with quality traits in durum wheat

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• 作者：Lamiae Amallah ; Mouna Taghouti ; Keltoum Rhrib…
• 刊名：Journal of Crop Science and Biotechnology
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：19
• 期：2
• 页码：137-150
• 全文大小：376 KB
• 刊物主题：Plant Sciences; Plant Genetics & Genomics; Plant Pathology; Plant Physiology; Biotechnology;
• 出版者：Springer Netherlands
• ISSN：2005-8276
• 卷排序：19

文摘

Over recent years, quality has become an important commercial issue for durum wheat breeders. Modern breeding methods are most efficient for producing and supplying the best quality raw materials to the pasta industry. Here we assessed the effectiveness of molecular marker-assisted selection of quality traits in durum wheat. To this end, DNA and quality trait markers were jointly used to analyze quality-related traits in a durum wheat collection. A total of 132 durum wheat (Triticum turgidum ssp. durum) Mediterranean landraces, international lines, and Moroccan cultivars were analyzed for seven important qualityrelated traits including thousand-kernel weight (TKW), test weight (TW), gluten strength, yellow pigment (YP), and grain protein content (GPC). Additionally, 18 simple sequence repeat (SSR) markers previously reported to be associated with different quality traits were analyzed. Of these, 14 (78%) were polymorphic and four were monomorphic. There were between two and seven alleles per locus, with an average of four alleles per locus. The average phenotypic variation value (R2) ranged from 2.81 to 20.43%. Association analysis identified nine markers significantly associated with TKW, TW, and YP, followed by eight markers associated with GPC, six markers associated with yellow index b, four markers associated with brightness L, and three markers associated with SDS-sedimentation volume. This study highlights the efficiency of SSR technology, which holds promise for a wide range of applications in marker-assisted wheat breeding programs.Keywordsmarker validationsimple sequence repeat (SSR)quality traitsTriticum turgidum sspDurum