Synthetic photosynthetic consortia define interactions leading to robustness and photoproduction
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- 作者：Stephanie G. Hays ; Leo L. W. Yan ; Pamela A. Silver…
- 关键词：Synthetic biology ; Photoproduction ; Synthetic consortia ; Microbial communities
- 刊名：Journal of Biological Engineering
- 出版年：2017
- 出版时间：December 2017
- 年：2017
- 卷：11
- 期：1
- 全文大小：1556KB
- 刊物类别：Engineering
- 刊物主题：Biomedical Engineering; Biotechnology; Biological Techniques; Nucleic Acid Chemistry; Applied Microbiology; Environmental Engineering/Biotechnology;
- 出版者：BioMed Central
- ISSN：1754-1611
- 卷排序：11
文摘
BackgroundMicrobial consortia composed of autotrophic and heterotrophic species abound in nature, yet examples of synthetic communities with mixed metabolism are limited in the laboratory. We previously engineered a model cyanobacterium, Synechococcus elongatus PCC 7942, to secrete the bulk of the carbon it fixes as sucrose, a carbohydrate that can be utilized by many other microbes. Here, we tested the capability of sucrose-secreting cyanobacteria to act as a flexible platform for the construction of synthetic, light-driven consortia by pairing them with three disparate heterotrophs: Bacillus subtilis, Escherichia coli, or Saccharomyces cerevisiae. The comparison of these different co-culture dyads reveals general design principles for the construction of robust autotroph/heterotroph consortia.