Proteins Associated with Oxidative Burst and Cell Wall Strengthening Accumulate During Citrus-Xanthomonas Non-Host Interaction

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• 作者：T. Swaroopa Rani ; Daisuke Takahashi ; Matsuo Uemura…
• 关键词：Proteome analysis ; Citrus ; Xanthomonas ; nano ; LC ; MS/MS ; 2DE ; Pathogens
• 刊名：Plant Molecular Biology Reporter
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：33
• 期：5
• 页码：1349-1360
• 全文大小：2,316 KB
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• 作者单位：T. Swaroopa Rani (1)
  Daisuke Takahashi (2)
  Matsuo Uemura (2)
  Appa Rao Podile (1)
  
  1. Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, 500 046, Telangana, India
  2. Cryobiofrontier Research Center and United Graduate School of Agricultural Sciences, Iwate University, Morioka, 020-8550, Japan
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  Plant Physiology
  
• 出版者：Springer Netherlands
• ISSN：1572-9818

文摘

Citrus proteome changes at 8 and 48 h post inoculation (hpi) were analysed by both 2D gel electrophoresis and nano-LC-MS/MS proteomic approaches during interaction with Xanthomonas axonopodis pv. citri (Xac) and Xanthomonas oryzae pv. oryzae (Xoo) as host and non-host pathogens, respectively. A total of 256 proteins, 72 at 8 hpi and 184 at 48 hpi, differentially accumulated during citrus-Xanthomonas interaction. Of these, 67 and 115 proteins were specific to Xac and Xoo interaction, respectively. In addition, 64 proteins, 10 at 8 hpi and 54 at 48 hpi, variedly accumulated during both the interactions. Proteins related to photosynthesis, carbohydrate metabolism and protein synthesis were in low abundance during both the interactions resulting in reduced rate of photosynthesis. Proteins related to defence response, cell wall (CW) strengthening, lignin deposition and generation of reactive oxygen species (ROS) were in high abundance only during Xoo interaction. Whereas, during Xac interaction, proteins involved in antioxidant metabolism and CW loosening and/or elongation were in high abundance. The precise increase in abundance of these proteins during non-host interaction suggested an important role for CW fortification and ROS accumulation in non-host resistance in plants. Keywords Proteome analysis Citrus Xanthomonas nano-LC-MS/MS 2DE Pathogens