Higher prevalence and levels of Nosema ceranae than Nosema apis infections in Canadian honey bee colonies

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• 作者：Berna Emsen ; Ernesto Guzman-Novoa ; Mollah Md Hamiduzzaman…
• 关键词：Nosema ceranae ; Nosema apis ; Microsporidia ; Apis mellifera ; Prevalence ; Virulence
• 刊名：Parasitology Research
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：115
• 期：1
• 页码：175-181
• 全文大小：391 KB
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• 作者单位：Berna Emsen (1) (2)
  Ernesto Guzman-Novoa (2)
  Mollah Md Hamiduzzaman (2)
  Les Eccles (3)
  Brian Lacey (3)
  Rosario A. Ruiz-Pérez (1) (4)
  Medhat Nasr (5)
  
  1. Department of Animal Science, Ataturk University, Erzurum, 25240, Turkey
  2. School of Environmental Sciences, University of Guelph, 50 Stone Road East, Guelph, Ontario, N1G 2W1, Canada
  3. Ontario Beekeepers Association, 5420 – 185 Hwy 6 N, Guelph, Ontario, N1H 6J2, Canada
  4. Departamento de Producción Animal: Abejas, FMVZ, UNAM, Cd. Univ, Mexico, D.F., 04510, Mexico
  5. Crop Diversification Centre North, Alberta Agriculture and Food, Edmonton, Alberta, T5Y 6H3, Canada
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Medical Microbiology
  Microbiology
  Immunology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-1955

文摘

This study was conducted to determine the prevalence and infection levels of the microsporidia fungi Nosema apis and/or Nosema ceranae in honey bee colonies of two Canadian provinces. Three surveys were conducted in the springs of 2008, 2010 and 2012 and PCR identification of Nosema species were performed in samples from 169 and 181 Ontario colonies and from 76 Alberta colonies that tested positive to Nosema spp. Infection levels of positive colonies were determined by microscopy and analyzed by Nosema spp. Results showed that N. ceranae was the dominant species in all three surveys (prevalence range of 41–91 vs. 4–34 % for N. apis), whereas mixed infections were less frequent than single infections (5–25 %). Infection levels of colonies parasitized by N. ceranae were three to five times higher than those of colonies parasitized by N. apis in the three surveys whereas mixed infections showed the highest spore counts. This is the first field study demonstrating significantly higher infection levels in colonies parasitized with either N. ceranae only or with both, N. ceranae and N. apis, than in colonies parasitized with N. apis only. Taken together, these results suggest that N. ceranae may be more virulent and better adapted than N. apis in cold climates such as Canadian environments.