(Teil-)Automatisierung der liquid-overlay-Sphäroidkultivierung

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• 作者：Alexander Krüger ; Mirjam Ingargiola ; Melanie Hüther ; Leoni A. Kunz-Schughart
• 刊名：BIOspektrum
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：22
• 期：5
• 页码：480-484
• 全文大小：642 KB
• 刊物主题：Life Sciences, general; Biochemistry, general; Human Genetics; Microbiology; Developmental Biology; Pharmacology/Toxicology;
• 出版者：Springer Berlin Heidelberg
• ISSN：1868-6249
• 卷排序：22

文摘

3D tumor spheroid models are increasingly applied in secondary, cellbased drug testing due to certain therapy-relevant in vivo-like characteristics. Automation of routine and delicate steps in spheroid culturing and processing, including the gentle well-to-well transfer of spheroids, facilitates spheroid-based assay standardization. Semi-automated workflows minimize experimental variations and are recommended in particular for combination treatment testing and long-term monitoring.