A rapid and cost-effective method for genotyping apolipoprotein E gene polymorphism

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• 作者：Li Zhong ; Yong-Zhuang Xie ; Tian-Tian Cao ; Zongqi Wang…
• 关键词：Apolipoprotein E ; APOE genotype ; Real Time PCR ; Sequencing ; TaqMan ; Polymorphism
• 刊名：Molecular Neurodegeneration
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：11
• 期：1
• 全文大小：1,921 KB
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• 作者单位：Li Zhong (1)
  Yong-Zhuang Xie (1)
  Tian-Tian Cao (1)
  Zongqi Wang (1)
  Tingting Wang (1)
  Xinxiu Li (1)
  Rui-Chi Shen (2)
  Huaxi Xu (1)
  Guojun Bu (1) (3)
  Xiao-Fen Chen (1)
  
  1. Fujian Provincial Key Laboratory of Neurodegenerative Disease and Aging Research, Institute of Neuroscience, Medical College, Xiamen University, Xiamen, 361102, China
  2. Fujian Institute of Subtropical Botany, Xiamen, 361006, China
  3. Department of Neuroscience, Mayo Clinic, Jacksonville, FL, 32224, USA
  
• 刊物主题：Neurosciences; Neurology; Molecular Medicine;
• 出版者：BioMed Central
• ISSN：1750-1326

文摘

Background Apolipoprotein E (ApoE) is a major cholesterol carrier and plays an important role in maintaining lipid homeostasis both in the periphery and brain. Human APOE gene is polymorphic at two single nucleotides (rs429358 and rs7412) resulting in three different alleles (ε2, ε3 and ε4). ApoE isoforms modulate the risk for a variety of vascular and neurodegenerative diseases; thus, APOE genotyping is crucial for predicting disease risk and designing individualized therapy based on APOE genotype.