Detection of DNA from Escherichia coli, Clostridium perfringens, Staphylococcus aureus and Bacillus cereus after simplified enrichment using a novel multiplex real-time PCR system
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- 作者：René Köppel ; Ingrid Tolido ; Gabriela Marti…
- 关键词：Multiplex real ; time quantitative PCR ; Escherichia coli ; Clostridium perfringens ; Staphylococcus aureus and Bacillus cereus ; Universal enrichment
- 刊名：European Food Research and Technology
- 出版年：2017
- 出版时间：March 2017
- 年：2017
- 卷：243
- 期：3
- 页码：521-530
- 全文大小：
- 刊物类别：Chemistry and Materials Science
- 刊物主题：Food Science; Analytical Chemistry; Biotechnology; Agriculture; Forestry;
- 出版者：Springer Berlin Heidelberg
- ISSN：1438-2385
- 卷排序：243
文摘
A novel multiplex real-time PCR called AllBaktB is presented for the simultaneous determination of DNA from Escherichia coli, Clostridium perfringens, Staphylococcus aureus and Bacillus cereus in food samples. This semi-automated procedure involves a simplified nonselective enrichment and a single isolation step for all bacterial DNA. The results can be gained within 24 h and correspond to the results obtained by the cultivation based on ISO methods. The achieved detection limit was estimated to be at 35 colony forming units per gram food sample for all analytes. A ring trial showed that the method can be transferred to other laboratories showing its robustness.