Zinc compartmentation in root, transport into xylem, and absorption into leaf cells in the hyperaccumulating species of Sedum alfredii Hance
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- 作者：Xiaoe Yang (1)
Tingqiang Li (1)
Juncheng Yang (2)
Zhenli He (1) (3)
Lingli Lu (1)
Fanhua Meng (1) - 关键词：Compartmentation ; Hyperaccumulator ; Organic and amino acids ; Protoplast ; Zinc
- 刊名：Planta
- 出版年：2006
- 出版时间：June 2006
- 年：2006
- 卷：224
- 期：1
- 页码：185-195
- 全文大小：325KB
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Tingqiang Li (1)
Juncheng Yang (2)
Zhenli He (1) (3)
Lingli Lu (1)
Fanhua Meng (1)
1. MOE Key Laboratory of Environmental Remediation and Ecosystem Health, College of Natural Resources and Environmental Sciences, Zhejiang University, Huajianchi Campus, 310029, Hangzhou, China
2. Soil and Fertilizer Institute, Chinese Academy of Agricultural Sciences, 100081, Beijing, China
3. Indian River Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida, 2199 South Rock Road, Fort Pierce, FL, 34945-3138, USA
文摘
Sedum alfredii Hance can accumulate Zn in shoots over 2%. Leaf and stem Zn concentrations of the hyperaccumulating ecotype (HE) were 24- and 28-fold higher, respectively, than those of the nonhyperaccumulating ecotype (NHE), whereas 1.4-fold more Zn was accumulated in the roots of the NHE. Approximately 2.7-fold more Zn was stored in the root vacuoles of the NHE, and thus became unavailable for loading into the xylem and subsequent translocation to shoot. Long-term efflux of absorbed 65Zn indicated that 65Zn activity was 6.8-fold higher in shoots but 3.7-fold lower in roots of the HE. At lower Zn levels (10 and 100?μM), there were no significant differences in 65Zn uptake by leaf sections and intact leaf protoplasts between the two ecotypes except that 1.5-fold more 65Zn was accumulated in leaf sections of the HE than in those of the NHE after exposure to 100?μM for 48?h. At 1,000?μM Zn, however, approximately 2.1-fold more Zn was taken up by the HE leaf sections and 1.5-fold more 65Zn taken up by the HE protoplasts as compared to the NHE at exposure times >16?h and >10?min, respectively. Treatments with carbonyl cyanide m-chlorophenylhydrazone (CCCP) or ruptured protoplasts strongly inhibited 65Zn uptake into leaf protoplasts for both ecotypes. Citric acid and Val concentrations in leaves and stems significantly increased for the HE, but decreased or had minimal changes for the NHE in response to raised Zn levels. These results indicate that altered Zn transport across tonoplast in the root and stimulated Zn uptake in the leaf cells are the major mechanisms involved in the strong Zn hyperaccumulation observed in S. alfredii H.