Preventive Effect of Hochu-ekki-to on Lipopolysaccharide-Induced Acute Lung Injury in BALB/c Mice
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- 作者：Shunji Tajima ; Masashi Bando ; Hideaki Yamasawa ; Shoji Ohno ; Hiroshi Moriyama ; Toshinori Takada ; Eiichi Suzuki ; Fumitake Gejyo and Yukihiko Sugiyama
- 关键词：Acute lung injury ; Granulocyte ; macrophage colony ; stimulating factor ; Hochu ; ekki ; to ; Keratinocyte chemoattractant ; Lipopolysaccharide
- 刊名：Lung
- 出版年：2006
- 出版时间：December, 2006
- 年：2006
- 卷：184
- 期：6
- 页码：318-323
- 全文大小：193 KB
- 刊物类别：Medicine
- 刊物主题：Medicine & Public Health
Pneumology and Respiratory System - 出版者：Springer New York
- ISSN：1432-1750
文摘
This study was designed to investigate the effect of Hochu-ekki-to (TJ-41), a Japanese herbal medicine, on the development of lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice. ALI was induced in female BALB/c mice by the intranasal administration of 0.1 mg/kg LPS. The mice were divided into a group receiving normal feed and another group receiving feed mixed with TJ-41 at a dose of 1 g/kg/day for 8 weeks before LPS challenge. In the bronchoalveolar lavage fluid, the preadministration of TJ-41 caused significant reduction in the absolute number of total cells, neutrophils, and macrophages. The preadministration of TJ-41 significantly inhibited increases in the serum level of keratinocyte chemoattractant (KC), which is a murine chemotaxin for neutrophils that corresponds to human interleukin-8, with respect to its concentration at 24 h after LPS challenge. Furthermore, the histopathologic findings indicated that alveolitis with leukocyte infiltration in the alveolar space was less severe in the TJ-41-treated mice than in the control mice. These findings indicated that the preadministration of TJ-41 could show an inhibitory effect on ALI in this experimental murine system associated with the suppression of chemokine production.