New PAH gene promoter KLF1 and 3′-region C/EBPalpha motifs influence transcription in vitro

详细信息    查看全文

• 作者：Kristel Klaassen ; Biljana Stankovic ; Nikola Kotur…
• 关键词：3′ Non ; coding region ; Phenylalanine hydroxylase gene ; Phenylketonuria ; Promoter ; Silencer ; Transcription
• 刊名：Journal of Applied Genetics
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：58
• 期：1
• 页码：79-85
• 全文大小：
• 刊物主题：Plant Genetics & Genomics; Human Genetics; Animal Genetics and Genomics; Microbial Genetics and Genomics;
• 出版者：Springer Berlin Heidelberg
• ISSN：2190-3883
• 卷排序：58

文摘

Phenylketonuria (PKU) is a metabolic disease caused by mutations in the phenylalanine hydroxylase (PAH) gene. Although the PAH genotype remains the main determinant of PKU phenotype severity, genotype–phenotype inconsistencies have been reported. In this study, we focused on unanalysed sequences in non-coding PAH gene regions to assess their possible influence on the PKU phenotype. We transiently transfected HepG2 cells with various chloramphenicol acetyl transferase (CAT) reporter constructs which included PAH gene non-coding regions. Selected non-coding regions were indicated by in silico prediction to contain transcription factor binding sites. Furthermore, electrophoretic mobility shift assay (EMSA) and supershift assays were performed to identify which transcriptional factors were engaged in the interaction. We found novel KLF1 motif in the PAH promoter, which decreases CAT activity by 50 % in comparison to basal transcription in vitro. The cytosine at the c.-170 promoter position creates an additional binding site for the protein complex involving KLF1 transcription factor. Moreover, we assessed for the first time the role of a multivariant variable number tandem repeat (VNTR) region located in the 3′-region of the PAH gene. We found that the VNTR3, VNTR7 and VNTR8 constructs had approximately 60 % of CAT activity. The regulation is mediated by the C/EBPalpha transcription factor, present in protein complex binding to VNTR3. Our study highlighted two novel promoter KLF1 and 3′-region C/EBPalpha motifs in the PAH gene which decrease transcription in vitro and, thus, could be considered as PAH expression modifiers. New transcription motifs in non-coding regions will contribute to better understanding of the PKU phenotype complexity and may become important for the optimisation of PKU treatment.