Isolation and Characterization of Microsatellite Markers for Viola websteri (Violaceae) and Cross-Species Amplification within the Genus Viola
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- 作者：Jong-Soo Kang ; Xianchun Zhang ; Myounghai Kwak
- 关键词：Viola websteri ; Viola ; Microsatellites ; Cross ; amplification ; Transferability
- 刊名：Plant Molecular Biology Reporter
- 出版年：2017
- 出版时间：February 2017
- 年：2017
- 卷：35
- 期：1
- 页码：145-153
- 全文大小：
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Plant Sciences; Plant Breeding/Biotechnology; Proteomics; Metabolomics; Bioinformatics;
- 出版者：Springer US
- ISSN：1572-9818
- 卷排序：35
文摘
We isolated and characterized microsatellite loci in Viola websteri (Violaceae), an endangered species from Korea and endemic to Northeast Asia. A total of 27 microsatellite loci were developed and tested in Korean and Chinese populations. The number of alleles per locus varied from two to eight. The observed and expected heterozygosities within two populations were 0.000 to 1.000 and 0.080 to 0.816, respectively. Korean and Chinese populations were clearly distinguished by the private alleles from 16 loci. A total of 21 loci out of the 27 developed loci were successfully cross-amplified in 39 other Viola species. We believe that these microsatellite loci will be useful for future studies on genetic diversity and population structure of V. websteri, as well as other Viola species.