Identification of genes affecting alginate biosynthesis in Pseudomonas fluorescens by screening a transposon insertion library
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- 作者：Helga Ertesvåg ; Håvard Sletta ; Mona Senneset ; Yi-Qian Sun ; Geir Klinkenberg…
- 关键词：Pseudomonas fluorescens ; Alginate biosynthesis ; Transposon mutants ; Fructose 6 ; phosphate ; Purine ; Tryptophan ; Peptidoglycan recycling
- 刊名：BMC Genomics
- 出版年：2017
- 出版时间：December 2017
- 年：2017
- 卷：18
- 期：1
- 全文大小：669KB
- 刊物主题：Life Sciences, general; Microarrays; Proteomics; Animal Genetics and Genomics; Microbial Genetics and Genomics; Plant Genetics & Genomics;
- 出版者：BioMed Central
- ISSN：1471-2164
- 卷排序：18
文摘
BackgroundPolysaccharides often are necessary components of bacterial biofilms and capsules. Production of these biopolymers constitutes a drain on key components in the central carbon metabolism, but so far little is known concerning if and how the cells divide their resources between cell growth and production of exopolysaccharides. Alginate is an industrially important linear polysaccharide synthesized from fructose 6-phosphate by several bacterial species. The aim of this study was to identify genes that are necessary for obtaining a normal level of alginate production in alginate-producing Pseudomonas fluorescens.