Downregulation of dTps1 in Drosophila melanogaster larvae confirms involvement of trehalose in redox regulation following desiccation

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• 作者：Leena Thorat ; Krishna-Priya Mani ; Pradeep Thangaraj…
• 关键词：Drosophila ; Desiccation stress ; Trehalose ; ROS ; SOD ; dTps1 ; RNAi transgene
• 刊名：Cell Stress and Chaperones
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：21
• 期：2
• 页码：285-294
• 全文大小：1,347 KB
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• 作者单位：Leena Thorat (1)
  Krishna-Priya Mani (2)
  Pradeep Thangaraj (2)
  Suvro Chatterjee (2) (3)
  Bimalendu B. Nath (1)
  
  1. UGC-Centre for Advanced Studies, Stress Biology Research Laboratory, Department of Zoology, Savitribai Phule Pune University, Pune, 411007, India
  2. AU-KBC Research Centre, MIT Campus of Anna University, Chennai, Tamil Nadu, 600044, India
  3. Department of Biotechnology, Anna University, Chennai, Tamil Nadu, 600044, India
  
• 刊物主题：Biomedicine general; Cell Biology; Biochemistry, general; Immunology; Cancer Research; Neurosciences;
• 出版者：Springer Netherlands
• ISSN：1466-1268

文摘

As a survival strategy to environmental water deficits, desiccation-tolerant organisms are commonly known for their ability to recruit stress-protective biomolecules such as trehalose. We have previously reported the pivotal role of trehalose in larval desiccation tolerance in Drosophila melanogaster. Trehalose has emerged as a versatile molecule, serving mainly as energy source in insects and also being a stress protectant. While several recent reports have revealed the unconventional role of trehalose in scavenging reactive oxygen species in yeast and plants, this aspect has not received much attention in animals. We examined the status of desiccation-induced generation of reactive oxygen species in D. melanogaster larvae and the possible involvement of trehalose in ameliorating the harmful consequences thereof. Insect trehalose synthesis is governed by the enzyme trehalose 6-phosphate synthase 1 (TPS1). Using the ubiquitous da-GAL4-driven expression of the dTps1-RNAi transgene, we generated dTps1-downregulated Drosophila larvae possessing depleted levels of dTps1 transcripts. This resulted in the inability of the larvae for trehalose synthesis, thereby allowing us to elucidate the significance of trehalose in the regulation of desiccation-responsive redox homeostasis. Furthermore, the results from molecular genetics studies, biochemical assays, electron spin resonance analyses and a simple, non-invasive method of whole larval live imaging suggested that trehalose in collaboration with superoxide dismutase (SOD) is involved in the maintenance of redox state in D. melanogaster.