Effect of high carbohydrate diet on elongase and desaturase activity and accompanying gene expression in rat’s liver

详细信息    查看全文

• 作者：Jagoda Drąg ; Anna Goździalska ; Małgorzata Knapik-Czajka ; Anna Gawędzka…
• 关键词：Elongases ; Desaturases ; Fatty acids ; High carbohydrate diet
• 刊名：Genes & Nutrition
• 出版年：2017
• 出版时间：December 2017
• 年：2017
• 卷：12
• 期：1
• 全文大小：648KB
• 刊物主题：Human Genetics; Clinical Nutrition; Gene Function; Biomedicine, general;
• 出版者：BioMed Central
• ISSN：1865-3499
• 卷排序：12

文摘

BackgroundHepatic fatty acids (FAs) are modified through different metabolic pathways including elongation and desaturation. These processes are catalyzed by elongases and desaturases, respectively. Glucose, by transcription factors, regulates these processes. The aim of the study was to evaluate the influence of high carbohydrate diet (68%) on the expression of elongase (Elovl-2, Elovl-5, and Elovl-6) and desaturase (∆5D, ∆6D, Scd 1, Scd 2) genes and the activity of the enzymes. The changes in serum lipid profile (triglycerides (TG), total cholesterol (TC), HDL cholesterol) and glucose concentration were measured. Male Wistar rats were randomized into two study groups: animals fed with high carbohydrate diet (n = 6; HiCHO) and a control group fed with a standard diet (n = 6; ST). The expression of mRNA was determinate using reverse transcription PCR (RT-PCR). Hepatic FA composition was determined by gas chromatography, and FA ratios were used to estimate the activity of enzymes. Serum lipid profile and glucose concentration were measured using spectrophotometric methods.