Comparative assessment of the bacterial communities associated with Aedes aegypti larvae and water from domestic water storage containers

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• 作者：Nsa Dada (1)
  Estelle Jumas-Bilak (2) (3)
  Sylvie Manguin (4)
  Razak Seidu (1)
  Thor-Axel Stenstr枚m (1) (5)
  Hans J Overgaard (1) (6) (7)
  
  1. Department of Mathematical Sciences and Technology ; Norwegian University of Life Sciences ; 脜s ; Norway
  2. Laboratoire d鈥橦ygi猫ne hospitali猫re ; Centre Hospitalier R茅gional Universitaire de Montpellier ; Montpellier ; France
  3. UMR5119 ; Universit茅 Montpellier 1 ; Montpellier ; France
  4. IRD2 Institut de Recherche pour le D茅veloppement (IRD) ; UMR-MD3 ; Universit茅 Montpellier 1 ; Montpellier ; France
  5. SARChl Chair ; Institute for Water and Waste Water Technology ; Durban University of Technology ; Durban ; South Africa
  6. Institut de Recherche pour le D茅veloppement (IRD) ; Maladies Infectieuses et Vecteurs ; Ecologie ; G茅n茅tique ; Evolution et Contr么le (IRD 224-CNRS 5290 UM1-UM2) ; Montpellier Cedex 5 ; France
  7. Department of Entomology ; Faculty of Agriculture ; Kasetsart University ; Bangkok ; 10900 ; Thailand
  
• 关键词：Aedes aegypti ; Bacterial diversity ; Enteric bacteria ; E. coli ; Domestic water storage containers ; 16S rRNA ; TTGE ; Thailand
• 刊名：Parasites & Vectors
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：7
• 期：1
• 全文大小：2,692 KB
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• 刊物主题：Parasitology; Infectious Diseases; Tropical Medicine; Entomology;
• 出版者：BioMed Central
• ISSN：1756-3305

文摘

Background Domestic water storage containers constitute major Aedes aegypti breeding sites. We present for the first time a comparative analysis of the bacterial communities associated with Ae. aegypti larvae and water from domestic water containers. Methods The 16S rRNA-temporal temperature gradient gel electrophoresis (TTGE) was used to identify and compare bacterial communities in fourth-instar Ae. aegypti larvae and water from larvae positive and negative domestic containers in a rural village in northeastern Thailand. Water samples were cultured for enteric bacteria in addition to TTGE. Sequences obtained from TTGE and bacterial cultures were clustered into operational taxonomic units (OTUs) for analyses. Results Significantly lower OTU abundance was found in fourth-instar Ae. aegypti larvae compared to mosquito positive water samples. There was no significant difference in OTU abundance between larvae and mosquito negative water samples or between mosquito positive and negative water samples. Larval samples had significantly different OTU diversity compared to mosquito positive and negative water samples, with no significant difference between mosquito positive and negative water samples. The TTGE identified 24 bacterial taxa, belonging to the phyla Proteobacteria, Firmicutes, Actinobacteria, Bacteroidetes and TM7 (candidate phylum). Seven of these taxa were identified in larval samples, 16 in mosquito positive and 13 in mosquito negative water samples. Only two taxa, belonging to the phyla Firmicutes and Actinobacteria, were common to both larvae and water samples. Bacilli was the most abundant bacterial class identified from Ae. aegypti larvae, Gammaproteobacteria from mosquito positive water samples, and Flavobacteria from mosquito negative water samples. Enteric bacteria belonging to the class Gammaproteobacteria were sparsely represented in TTGE, but were isolated from both mosquito positive and negative water samples by selective culture. Conclusions Few bacteria from water samples were identified in fourth-instar Ae. aegypti larvae, suggesting that established larval bacteria, most likely acquired at earlier stages of development, control the larval microbiota. Further studies at all larval stages are needed to fully understand the dynamics involved. Isolation of enteric bacteria from water samples supports earlier outcomes of E. coli contamination in Ae. aegypti infested domestic containers, suggesting the need to further explore the role of enteric bacteria in Ae. aegypti infestation.