Magnetic microparticle-based SELEX process for the identification of highly specific aptamers of heart marker--brain natriuretic peptide
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- 作者：Ying Wang (1) (2)
Jingjing Wu (2)
Yinji Chen (3)
Feng Xue (2)
Jun Teng (2)
Jinxuan Cao (1)
Chunxia Lu (4)
Wei Chen (2)
1. Department of Materials Science and Chemical Engineering ; Ningbo University ; No 818# ; Fenghua Road ; Ningbo ; 315211 ; People鈥檚 Republic of China
2. School of Biotechnology and Food Engineering ; Anhui Provincial Key Lab of Functional Materials & Devices ; Hefei University of Technology ; Hefei ; 23009 ; People鈥檚 Republic of China
3. National Engineering Lab of Food Storage and Transportation ; Nanjing University of Finances and Economics ; Nanjing ; 210023 ; People鈥檚 Republic of China
4. Xinjiang Academy of Agricultral And Reclamation Science ; Xinjiang ; China - 关键词：SELEX ; Magnetic microparticle ; Aptamers ; Brain natriuretic peptide (BNP) ; Rapid and early diagnosis
- 刊名：Microchimica Acta
- 出版年：2015
- 出版时间：January 2015
- 年：2015
- 卷：182
- 期：1-2
- 页码：331-339
- 全文大小：862 KB
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- 刊物主题：Chemistry
Analytical Chemistry
Inorganic Chemistry
Physical Chemistry
Characterization and Evaluation Materials
Monitoring, Environmental Analysis and Environmental Ecotoxicology - 出版者：Springer Wien
- ISSN：1436-5073
文摘
The brain natriuretic peptide (BNP) is known to be an effective indicator of heart failure. It has been widely adopted as a parameter for the evaluation of heart function of cardiovascular and cerebrovascular diseases (CVDs). Current immune-recognition based methods for the detection of BNP are limited, to a certain extent, by the poor stability of the antibody and by high costs. The availability of an aptamer specific for BNP would greatly assist in the rapid and early diagnosis of CVDs. In order to screen for such an aptamer by the SELEX method, we have used magnetic microparticles (m-MPs) as the separation substrate for immobilization of target BNP. The use of m-MPs for rapid separation of combined aptamers enables bound oligonucleotides to be separated directly, quickly, and with high efficiency. After 14 rounds of selection, a panel of six aptamers against BNP was identified. Their dissociation constants range from 12.5 to 139 nM. The classical technique for conjugation of a target to m-MPs is known to be applicable to various fields, and we conclude that this m-MP-based SELEX process provides a general strategy for screening of specific aptamers against various analytes. Graphical Abstract Rapid selection of aptamer to brain natriuretic peptide (BNP) with high affinity and specificity for rapid detection and daily monitoring of heart functions with the magnetic microparticles-based screening platform.