The Detection of Inherent Homologous Recombination Between Repeat Sequences in H. pylori 26695 by the PCR-Based Method
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- 作者：Yajuan Fu (1) (2)
Reyna Cristina Zepeda-Gurrola (1)
Germán Rubén Aguilar-Gutiérrez (3)
Edgar E. Lara-Ramírez (1)
Erick J. De Luna-Santillana (1)
Isabel Cristina Rodríguez-Luna (1)
Alejandro Sánchez-Varela (1)
Ricardo Carre?o-López (4)
Víctor Ricardo Moreno-Medina (1)
Mario A. Rodríguez-Pérez (1)
Yolanda López-Vidal (5)
Xianwu Guo (1) - 刊名：Current Microbiology
- 出版年：2014
- 出版时间：February 2014
- 年：2014
- 卷：68
- 期：2
- 页码：211-219
- 全文大小：279 KB
- 作者单位：Yajuan Fu (1) (2)
Reyna Cristina Zepeda-Gurrola (1)
Germán Rubén Aguilar-Gutiérrez (3)
Edgar E. Lara-Ramírez (1)
Erick J. De Luna-Santillana (1)
Isabel Cristina Rodríguez-Luna (1)
Alejandro Sánchez-Varela (1)
Ricardo Carre?o-López (4)
Víctor Ricardo Moreno-Medina (1)
Mario A. Rodríguez-Pérez (1)
Yolanda López-Vidal (5)
Xianwu Guo (1)
1. Centro de Biotecnología Genómica, Instituto Politécnico Nacional, Boulevard del Maestro S/N esq. Elías Pi?a, Col. Narciso Mendoza, Cd., 88710, Reynosa, Tamaulipas, Mexico
2. Centro de Investigación en Ciencia Aplicada y Tecnología Avanzada, Instituto Politécnico Nacional, Altamira, Tamaulipas, Mexico
3. Centro de Investigación sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Cuernavaca, Morelos, Mexico
4. Centro de Investigaciones en Ciencias Microbiológicas, Benemérita Universidad Autónoma de Puebla, Puebla, Mexico
5. Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, D.F., Mexico - ISSN：1432-0991
文摘
Helicobacter pylori infects more than half of the world’s population, making it the most widespread infection of bacteria. It has high genetic diversity and has been considered as one of the most variable bacterial species. In the present study, a PCR-based method was used to detect the presence and the relative frequency of homologous recombination between repeat sequences (>500?bp) in H. pylori 26695. All the recombinant structures have been confirmed by sequencing. The inversion generated between inverted repeats showed distinct features from the recombination for duplication or deletion between direct repeats. Meanwhile, we gave the mathematic reasoning of a general formula for the calculation of relative recombination frequency and indicated the conditions for its application. This formula could be extensively applied to detect the frequency of homologous recombination, site-specific recombination, and other types of predictable recombination. Our results should be helpful for better understanding the genome evolution and adaptation of bacteria.