TDP-43 protein variants as biomarkers in amyotrophic lateral sclerosis
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- 作者：Stephanie M. Williams ; Galam Khan ; Brent T. Harris ; John Ravits…
- 关键词：Amyotrophic lateral sclerosis ; TDP ; 43 variants ; Plasma ; Biomarker ; Brain tissue ; scFv
- 刊名：BMC Neuroscience
- 出版年：2017
- 出版时间：December 2017
- 年：2017
- 卷：18
- 期：1
- 全文大小：1730KB
- 刊物主题：Neurosciences; Neurobiology; Animal Models;
- 出版者：BioMed Central
- ISSN：1471-2202
- 卷排序：18
文摘
BackgroundTDP-43 aggregates accumulate in individuals affected by amyotrophic lateral sclerosis (ALS) and other neurodegenerative diseases, representing potential diagnostic and therapeutic targets. Using an atomic force microscopy based biopanning protocol developed in our lab, we previously isolated 23 TDP-43 reactive antibody fragments with preference for human ALS brain tissue relative to frontotemporal dementia, a related neurodegeneration, and healthy samples from phage-displayed single chain antibody fragment (scFv) libraries. Here we further characterize the binding specificity of these different scFvs and identify which ones have promise for detecting ALS biomarkers in human brain tissue and plasma samples.