Simultaneous analyses of N-linked and O-linked glycans of ovarian cancer cells using solid-phase chemoenzymatic method
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- 作者：Shuang Yang ; Naseruddin Höti ; Weiming Yang ; Yang Liu ; Lijun Chen…
- 关键词：Chemoenzymatic ; Glycoprotein ; Glycomics ; Solid phase
- 刊名：Clinical Proteomics
- 出版年：2017
- 出版时间：December 2017
- 年：2017
- 卷：14
- 期：1
- 全文大小：2744KB
- 刊物主题：Cell Biology; Pathology; Biotechnology; Proteomics;
- 出版者：BioMed Central
- ISSN：1559-0275
- 卷排序：14
文摘
BackgroundGlycans play critical roles in a number of biological activities. Two common types of glycans, N-linked and O-linked, have been extensively analyzed in the last decades. N-glycans are typically released from glycoproteins by enzymes, while O-glycans are released from glycoproteins by chemical methods. It is important to identify and quantify both N- and O-linked glycans of glycoproteins to determine the changes of glycans.