Influence of Graft Density of Poly (N-Isopropylacrylamide)-Grafted Silica on Separation Performance

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• 作者：Yanli Liang ; Zongjian Liu ; Rongji Dai ; Weiwei Meng ; Yulin Deng
• 关键词：N ; Isopropylacrylamide ; Retention behavior ; Graft densities ; ATRP
• 刊名：Chromatographia
• 出版年：2015
• 出版时间：November 2015
• 年：2015
• 卷：78
• 期：21-22
• 页码：1349-1357
• 全文大小：1,237 KB
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• 作者单位：Yanli Liang (1)
  Zongjian Liu (2)
  Rongji Dai (1)
  Weiwei Meng (1)
  Yulin Deng (1)
  
  1. The School of Life Science, Beijing Institute of Technology, Beijing, 100081, China
  2. Beijing Luohe Hospital, Capital Medical University, Beijing, 101100, China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Organic Chemistry
  Pharmacy
  Biochemistry
  Plant Sciences
  Measurement Science and Instrumentation
  
• 出版者：Vieweg Verlag
• ISSN：1612-1112

文摘

Poly (N-isopropylacrylamide) (PNIPAAm)-grafted silica beads with variable graft densities were prepared by atom transfer radical polymerization (ATRP). The ATRP initiator on the surface of silica was prepared through the aminopropyl silica and 2-bromoisobutyryl bromide. Acetyl chloride was used to control the graft density. The content of carbon, hydrogen, and nitrogen were measured by elemental analyzer. The graft ratio was characterized by thermogravimetric analysis and the molecular weight of the polymer was determined by GPC analysis. The retention behavior of five steroids under different temperature was studied. Furthermore, the influence of temperature and graft densities to the separation of steroid compounds was investigated. The results indicated that graft density had a significant influence on the separation efficiency. Thinner PNIPAAm chains on hydrophilic silica surface can achieve the stationary phase with enhanced temperature sensitivity and better separation property.