Characterization of hemangioblast in umbilical arteries of mid-gestation mouse embryos
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- 作者：Weixi Niu (1) (2)
He Huang (3)
Liangyu Zhao (4)
Zhuan Li (5)
Wenyan He (5)
Bing Liu (5)
Longgui Li (1)
Jiaxiang Xiong (6) - 关键词：Hemangioblast ; AGM ; Umbilical arteries ; Hematopoietic stem cells ; Endothelial cells
- 刊名：International Journal of Hematology
- 出版年：2012
- 出版时间：June 2012
- 年：2012
- 卷：95
- 期：6
- 页码：632-639
- 全文大小：733KB
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He Huang (3)
Liangyu Zhao (4)
Zhuan Li (5)
Wenyan He (5)
Bing Liu (5)
Longgui Li (1)
Jiaxiang Xiong (6)
1. Department of Cardiology, Xinqiao Hospital, Third Military Medical University, Chongqing, 400038, China
2. Department of Cardiology, The 309th Hospital of PLA, Beijing, 100091, China
3. Department of Anesthesiology, Xinqiao Hospital, Third Military Medical University, Chongqing, 400038, China
4. Department of Orthopaedics, Changzheng Hospital, Second Military Medical University, Shanghai, 200003, China
5. Laboratory of Oncology, Affiliated Hospital of Academy of Military Medical Sciences, Beijing, 100071, China
6. Department of Physiology, Third Military Medical University, Chongqing, 400038, China
文摘
Hemangioblasts are the common precursors of hematopoietic and vascular cells, and are characterized as blast colony-forming cells (BL-CFCs) in vitro. We previously identified BL-CFCs in the mouse aorta–gonads–mesonephros (AGM) region, but not yolk sac, placenta, circulation, or fetal liver. Here, we aim to determine whether BL-CFCs develop in the umbilical arteries (UA) that link the dorsal aorta (sub-region of AGM) and placenta. We find that the UA cells of E11.5 mouse embryos were capable of generating typical blast colonies. On replating, these colonies produced erythroid/myeloid progenitors and B220+ B lymphocytes in vitro, corroborating their definitive hematopoietic nature. They also generated CD31+ or endomucin+ tube-like structures on OP9 stromal cells, showing their endothelial potential. The proximal and distal regions of UA had equal numbers of BL-CFCs. To evaluate whether BL-CFCs can be autonomously maintained or expanded in UA or AGM, in vitro organ culture was performed. Interestingly, the BL-CFC pool in the AGM was significantly amplified, in striking contrast to a decrease in the UA. Taken together, our findings indicate that in addition to the AGM the UA serves as an important, but less supportive, niche for hemangioblast development.