Analysis of variable sites between two complete South China tiger (Panthera tigris amoyensis) mitochondrial genomes

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• 作者：Wenping Zhang (1) zhang_zoology@163.com
  Bisong Yue (2) bsyue@yahoo.com
  Xiaofang Wang (2)
  Xiuyue Zhang (2)
  Zhong Xie (3)
  Nonglin Liu (3)
  Wenyuan Fu (4)
  Yaohua Yuan (5)
  Daqing Chen (6)
  Danghua Fu (7)
  Bo Zhao (8)
  Yuzhong Yin (9)
  Xiahui Yan (10)
  Xinjing Wang (11)
  Rongying Zhang (12)
  Jie Liu (13)
  Maoping Li (14)
  Yao Tang (15)
  Rong Hou (1)
  Zhihe Zhang (1) zhangzhh@mail.sc.cninfo.net
• 关键词：Complete mitochondrial genome – ; Panthera tigris amoyensis – ; Variable site
• 刊名：Molecular Biology Reports
• 出版年：2011
• 出版时间：October 2011
• 年：2011
• 卷：38
• 期：7
• 页码：4257-4264
• 全文大小：264.5 KB
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• 作者单位：1. The Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife, Chengdu Research Base of Giant Panda Breeding, Chengdu, 610081 Sichuan, People鈥檚 Republic of China2. The Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife, Sichuan University, Chengdu, 610064 People鈥檚 Republic of China3. Chinese Association of Zoological Gardens, Beijing, China4. Fujian Meihuashan Institute of South China Tiger Breeding, Shanghang, China5. Shanghai Zoo, Shanghai, China6. Suzhou Zoo, Suzhou, China7. Nanchang Zoo, Nanchang, China8. Chengdu Zoo, Chengdu, China9. Chongqing Zoo, Chongqing, China10. Changsha Zoo, Changsha, China11. Guangzhou Zoo, Guangzhou, China12. Shenzhen Wild Zoo, Shenzhen, China13. Guiyang Wild Zoo, Guiyang, China14. Luoyang Wangcheng Garden, Luoyang, China15. Fuzhou Zoo, Fuzhou, China
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Animal Anatomy, Morphology and Histology
  Animal Biochemistry
  
• 出版者：Springer Netherlands
• ISSN：1573-4978

文摘

In order to investigate the mitochondrial genome of Panthera tigris amoyensis, two South China tigers (P25 and P27) were analyzed following 15 cymt-specific primer sets. The entire mtDNA sequence was found to be 16,957 bp and 17,001 bp long for P25 and P27 respectively, and this difference in length between P25 and P27 occurred in the number of tandem repeats in the RS-3 segment of the control region. The structural characteristics of complete P. t. amoyensis mitochondrial genomes were also highly similar to those of P. uncia. Additionally, the rate of point mutation was only 0.3% and a total of 59 variable sites between P25 and P27 were found. Out of the 59 variable sites, 6 were located in 6 different tRNA genes, 6 in the 2 rRNA genes, 7 in non-coding regions (one located between tRNA-Asn and tRNA-Tyr and six in the D-loop), and 40 in 10 protein-coding genes. COI held the largest amount of variable sites (9 sites) and Cytb contained the highest variable rate (0.7%) in the complete sequences. Moreover, out of the 40 variable sites located in 10 protein-coding genes, 12 sites were nonsynonymous.