Effects and mechanisms of resveratrol on the amelioration of oxidative stress and hepatic steatosis in KKAy mice

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• 作者：Wei Zhu (3)
  Sifan Chen (1) (2) (3)
  Zilun Li (4)
  Xiaohong Zhao (5)
  Wenxue Li (3)
  Yanshuang Sun (1) (2)
  Zili Zhang (1) (2)
  Wenhua Ling (1) (2)
  Xiang Feng (1) (2)
  
  3. Guangzhou Center for Disease Control and Prevention ; Guangzhou ; Guangdong ; People鈥檚 Republic of China
  1. School of Public Health ; Sun Yat-Sen University ; Guangzhou ; Guangdong ; People鈥檚 Republic of China
  2. Guangdong Provincial Key Laboratory of Food ; Nutrition and Health ; Guangzhou ; Guangdong ; People鈥檚 Republic of China
  4. Division of Vascular Surgery ; The First Affiliated Hospital ; Sun Yat-sen University ; Guangzhou ; Guangdong ; People鈥檚 Republic of China
  5. Department of Nephrology ; The First Affiliated Hospital ; Sun Yat-sen University ; Guangzhou ; Guangdong ; People鈥檚 Republic of China
  
• 关键词：NAFLD ; Lipid metabolic disorder ; Oxidative stress ; Resveratrol ; Sirt1 ; AMPK
• 刊名：Nutrition & Metabolism
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：11
• 期：1
• 全文大小：1,481 KB
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• 刊物类别：Chemistry and Materials Science
• 刊物主题：Nutrition
  Metabolic Diseases
  Clinical Nutrition
  
• 出版者：BioMed Central
• ISSN：1743-7075

文摘

Background The exact mechanism of the protective role of Resveratrol (Res) in lipid metabolism and oxidative stress is not well elucidated. The present study aimed to investigate the potential benefits and possible mechanisms of Res on the amelioration of oxidative stress and hepatic steatosis in a KKAy mouse model. Methods A total of 30 KKAy male mice were randomly divided into three groups: a normal chow group, a low resveratrol group and a high resveratrol group. After a 12-wk study period, serum levels of TG, TC, LDL-C and HDL-C, the liver content of TG and TC, ROS, GSH, GPx, SOD and MDA levels were measured. Ectopic lipid deposition was observed in sectioned frozen liver tissues. The mRNA levels of ATGL and HSL in the liver tissues were determined via real-time PCR. Furthermore, the protein expression of p47phox, gp91phox, ATGL, HSL, Sirt1, AMPK and FOXO1 were analyzed using western blotting. Results Following Res supplementation, serum levels of TG and MDA were decreased, while the HDL-C and SOD levels were increased in KKAy mice. Furthermore, Res treatment increased GSH and GPx in liver tissues, while it decreased ROS. In addition, Res significantly reduced hepatic steatosis. After Res treatment, concentrations of p47phox (membrane) and gp91phox proteins were reduced, while p-HSL, HSL and ATGL protein expression levels were increased. Mechanistically, the levels of Sirt1, p-AMPK and p-FOXO1 expression in the liver tissues were up-regulated following supplementation with Res, and FOXO1 protein was released from the nucleus into the cytoplasm. Conclusions Res is able to attenuate hepatic steatosis and lipid metabolic disorder and enhance the antioxidant ability in KKAy mice, possibly by up-regulating Sirt1 expression and the phosphorylation of AMPK.