Heat-killed yogurt-containing lactic acid bacteria prevent cytokine-induced barrier disruption in human intestinal Caco-2 cells

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• 作者：Juan Zeng ; Jinjiao Jiang ; Wenying Zhu ; Yufeng Chu
• 关键词：Probiotics ; ZO ; 1 ; Occludin
• 刊名：Annals of Microbiology
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：66
• 期：1
• 页码：171-178
• 全文大小：2,085 KB
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• 作者单位：Juan Zeng (1)
  Jinjiao Jiang (1)
  Wenying Zhu (1)
  Yufeng Chu (1)
  
  1. Department of Medical Intensive Care Unit, Shandong Provincial Hospital affiliated to Shandong University, Jingwu Road 324#, Jinan, Shandong, 250021, People’s Republic of China
  
• 刊物主题：Microbiology; Microbial Genetics and Genomics; Microbial Ecology; Fungus Genetics; Medical Microbiology; Applied Microbiology;
• 出版者：Springer Berlin Heidelberg
• ISSN：1869-2044

文摘

Probiotics have been proven to play a protective role against gut barrier disruption induced by enteroinvasive pathogens and chemical substances. To test the hypothesis that a heat-inactivated multi-strain of yogurt-containing lactic acid bacteria (Mix-LAB) (Lactobacillus bulgaricus, Streptococcus thermophilus, Lactobacillus acidophilus) could prevent epithelial barrier dysfunction induced by inflammatory cytokines, human intestinal Caco-2 cell monolayers were cultured for 72 h with cytomix (interferon-gamma, tumor necrosis factor-alpha, and interleukin-1 beta) alone and in combination with graded concentrations of heat-inactivated Mix-LAB (107,108,109/ml). After incubation for 72 h, cytomix decreased the transepithelial electrical resistance (TEER) and increased the paracellular permeability of Caco-2 monolayers to fluoresceinated dextran (molecular weight 376 Da), which was inhibited by the pretreatment of heat-inactivated Mix-LAB under culture conditions. This protective effect of heat-inactivated Mix-LAB on reduced TEER and increased paracellular permeability was concentration-dependent, rising to statistically significant levels for concentrations equal to or greater than 107 /ml after 72-h incubation and 108 /ml after 48-h incubation. Cytomix altered the expression and distribution of the tight junction proteins ZO-1 and occludin, but this effect was prevented by the heat-inactivated Mix-LAB. These data suggest that heat-inactivated yogurt-containing Mix-LAB plays a protective role in proinflammatory cytokine-induced intestinal epithelial barrier dysfunction. Keywords Probiotics ZO-1 Occludin