A novel esterase from a marine mud metagenomic library for biocatalytic synthesis of short-chain flavor esters
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- 作者：Wenyuan Gao ; Kai Wu ; Lifeng Chen ; Haiyang Fan ; Zhiqiang Zhao…
- 关键词：Metagenomic library ; Functional screening ; Esterase ; Transesterification ; Short ; chain flavor esters ; High substrate loading
- 刊名：Microbial Cell Factories
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：15
- 期：1
- 全文大小：2,663 KB
- 刊物类别：Chemistry and Materials Science
- 刊物主题：Biotechnology
Applied Microbiology
Environmental Engineering/Biotechnology - 出版者：BioMed Central
- ISSN：1475-2859
文摘
Background Marine mud is an abundant and largely unexplored source of enzymes with unique properties that may be useful for industrial and biotechnological purposes. However, since most microbes cannot be cultured in the laboratory, a cultivation-independent metagenomic approach would be advantageous for the identification of novel enzymes. Therefore, with the objective of screening novel lipolytic enzymes, a metagenomic library was constructed using the total genomic DNA extracted from marine mud.