The Role of Phospholipase D1 in Liver Fibrosis Induced by Dimethylnitrosamine In Vivo

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• 作者：Xinyan Zhu (1)
  Ruilin Liu (2)
  Dapeng Kuang (1)
  Jingqi Liu (1)
  Xiaomeng Shi (1)
  Tingting Zhang (1)
  Yu Zeng (3)
  Xianghua Sun (4)
  Yi Zhang (4)
  Wenzhuo Yang (1) (5)
  
• 关键词：Liver fibrosis ; Phospholipase D ; Dimethylnitrosamine ; N ; methylethanolamine
• 刊名：Digestive Diseases and Sciences
• 出版年：2014
• 出版时间：August 2014
• 年：2014
• 卷：59
• 期：8
• 页码：1779-1788
• 全文大小：4,196 KB
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• 作者单位：Xinyan Zhu (1)
  Ruilin Liu (2)
  Dapeng Kuang (1)
  Jingqi Liu (1)
  Xiaomeng Shi (1)
  Tingting Zhang (1)
  Yu Zeng (3)
  Xianghua Sun (4)
  Yi Zhang (4)
  Wenzhuo Yang (1) (5)
  
  1. Department of Gastroenterology and Digestive diseases Institute, Shanghai Tongji Hospital, Tongji University School of Medicine, Shanghai, 200065, China
  2. Department of Respiratory Diseases, Shanghai Tongji Hospital, Tongji University School of Medicine, Shanghai, 200065, China
  3. Department of Pathology, Shanghai Tongji Hospital, Tongji University School of Medicine, Shanghai, 200065, China
  4. Department of Central Laboratory, Shanghai Tongji Hospital, Tongji University School of Medicine, Shanghai, 200065, China
  5. Department of Gastroenterology and Digestive Diseases Institute, Shanghai Tongji Hospital, Tongji University School of Medicine, 389 Xincun Road, Shanghai, 200062, China
  
• ISSN：1573-2568

文摘

Background Phospholipase D (PLD) has been proved to be involved in regulating function of fibroblasts and might play a role in mediating organic fibrosis. Aims To investigate the role and mechanism of PLD on dimethylnitrosamine (DMN)-induced rat liver fibrosis. Methods Fifty-five male Wistar rats were divided into normal control group, DMN model group, N-methylethanolamine (MEA) control group, and MEA-intervention group. We observed the effects of MEA, a PLD inhibitor on the development and progression of rat liver fibrosis by comparing the physical and biochemical indexes, tissue pathology, PLD activity, and typical markers and cytokines related to fibrosis in the four groups. Results Accompanied by the down-regulation of PLD1 expression, the MEA-intervention group had improved outcomes compared with the DMN model group in terms of spleen weight, spleen/weight index, serum and tissue biochemical indexes, tissue hydroxyproline, and tissue pathology. The MEA-intervention group had lower TIMP1, COL1A1, and higher MMPs expression level than the DMN model group. The activity of PLD and PLD1, α-SMA expression level in the MEA-intervention group was much lower than those in the DMN model group. There was no significant difference between the two groups in the expression level of TGF-β1 and MCP1. Meanwhile, there were no significant differences between normal control group and MEA control group in the parameters stated above. Conclusion Phospholipase D1 may play an important role in the development and progression of rat liver fibrosis. Inhibition of PLD may become a new strategy to prevent or alleviate liver fibrosis.