Prevention of epimerization and quantitative determination of amygdalin in Armeniacae Semen with Schizandrae Fructus solution

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• 作者：Woo-Sang Joo (1)
  Ji-Seon Jeong (1)
  Hyogeun Kim (1)
  Yong-Moon Lee (2)
  Je-Hyun Lee (3)
  Seon-Pyo Hong (1)
  
• 关键词：Armeniacae Semen powder ; Schizandrae Fructus solution ; D ; Amygdalin ; Neoamygdalin ; Epimerization inhibition ; Conversion ratio ; Ma ; whang ; tang
• 刊名：Archives of Pharmacal Research
• 出版年：2006
• 出版时间：December 2006
• 年：2006
• 卷：29
• 期：12
• 页码：1096-1101
• 全文大小：695KB
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• 作者单位：Woo-Sang Joo (1)
  Ji-Seon Jeong (1)
  Hyogeun Kim (1)
  Yong-Moon Lee (2)
  Je-Hyun Lee (3)
  Seon-Pyo Hong (1)
  
  1. Department of Oriental Pharmaceutical Sciences, Kyung Hee University, 130-701, Seoul, Korea
  2. College of Pharmacy, Chungbuk National University, 361-763, Chongju, Korea
  3. Department of Korean Medicine, Dongguk University, Seokjang-dong, 780-714, Geongju-si, Korea
  
• ISSN：1976-3786

文摘

Armeniacae Semen not only contains amygdalin, but emulsin also, which is an enzyme that hydrolyzes amygdalin. The extraction yield of amygdalin from Armeniacae Semen was low, due to the presence of emulsin, when extracted with water. When Schizandrae Fructus solution was used as the extractant; however, amygdalin was almost completely extracted, regard-less of the cutting size due to the absence of the influence of emulsin. In addition, when the crude powder or small piece forms were used with Schizandrae Fructus solution, on epimerization of the D-amygdalin into neoamygdalin occurred. D-amygdalin and its conversion product, neoamygdalin, were quantitatively analyzed by reverse-phase, high-performance liquid chromatography (HPLC), with an optimized eluent of 10 mM sodium phosphate buffer (pH 2.3), containing 11.5% acetonitrile. The concentration and detector response were linearly correlated over the range 0.05 to 2 mM. The detection limits for both D-amygdalin and neoamygdalin were approximately 5 μM for the amount injected.