Discovery of herpesviruses in Canadian wildlife

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• 作者：Chimoné S. Dalton ; Karen van de Rakt ; Åsa Fahlman…
• 刊名：Archives of Virology
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：162
• 期：2
• 页码：449-456
• 全文大小：
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Virology; Medical Microbiology; Infectious Diseases;
• 出版者：Springer Vienna
• ISSN：1432-8798
• 卷排序：162

文摘

Herpesviruses (HVs) have a wide range of hosts in the animal kingdom. The result of infection with HVs can vary from asymptomatic to fatal diseases depending on subtype, strain, and host. To date, little is known about HVs naturally circulating in wildlife species and the impact of these viruses on other species. In our study, we used genetic and comparative approaches to increase our understanding of circulating HVs in Canadian wildlife. Using nested polymerase chain reaction targeting a conserved region of the HV DNA polymerase gene, we analyzed material derived from wildlife of western and northern Canada collected between February 2009 and Sept 2014. For classification of new virus sequences, we compared our viral sequences with published sequences in GenBank to identify conserved residues and motifs that are unique to each subfamily, alongside phylogenetic analysis. All alphaherpesviruses shared a conserved tryptophan (W856) and tyrosine (Y880), betaherpesviruses all shared a serine (S836), and gammaherpesviruses had a conserved glutamic acid (E835). Most of our wildlife HV sequences grouped together with HVs from taxonomically related host species. From Martes americana, we detected previously uncharacterized alpha- and beta-herpesviruses.