Isolation of prawn (Exopalaemon carinicauda) lipopolysaccharide and β-1, 3-glucan binding protein gene and its expression in responding to bacterial and viral infections

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• 作者：Qianqian Ge ; Jian Li ; Yafei Duan ; Jitao Li…
• 关键词：Exopalaemon carinicauda ; LGBP ; WSSV ; Vibrio parahaemolyticus ; immune response
• 刊名：Journal of Ocean University of China
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：15
• 期：2
• 页码：288-296
• 全文大小：1,040 KB
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• 作者单位：Qianqian Ge (1) (2) (4)
  Jian Li (2) (4)
  Yafei Duan (3)
  Jitao Li (1)
  Ming Sun (1) (2) (4)
  Fazhen Zhao (2) (4)
  
  1. Fisheries College, Ocean University of China, Qingdao, 266003, P. R. China
  2. Key Laboratory for Sustainable Utilization of Marine Fisheries Resources of Ministry of Agriculture, Chinese Academy of Fishery Sciences, Qingdao, 266071, P. R. China
  4. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, P. R. China
  3. Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization of Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510300, P. R. China
  
• 刊物主题：Oceanography; Meteorology;
• 出版者：Springer Berlin Heidelberg
• ISSN：1993-5021

文摘

The pattern recognition proteins (PRPs) play a major role in immune response of crustacean to resist pathogens. In the present study, as one of PRPs, lipopolysaccharide and β-1, 3-glucan binding protein (LGBP) gene in the ridge tail white prawn (Exopalaemon carinicauda) (EcLGBP) was isolated. The full-length cDNA of EcLGBP was 1338 bp, encoding a polypeptide of 366 amino acid residules. The deduced amino acid sequence of EcLGBP shared high similarities with LGBP and BGBP from other crustaceans. Some conservative domains were predicted in EcLGBP sequence. EcLGBP constitutively expressed in most tissues at different levels, and the highest expression was observed in hepatopancreas. With infection time, the cumulative mortality increased gradually followed by the proliferation of Vibrio parahaemolyticus and white spot syndrome virus (WSSV). The expression of EcLGBP in response to V. parahaemolyticus infection was up-regulated in hemocytes and hepatopancreas, and the up-regulation in hepatopancreas was earlier than that in hemocytes. EcLGBP expression after WSSV infection increased at 3 h, then significantly decreased in both hemocytes and hepatopancreas. The results indicated that EcLGBP was involved in the immune defense against bacterial and viral infections. Keywords Exopalaemon carinicauda LGBP WSSV Vibrio parahaemolyticus immune response