Role of Autophagy in Capsaicin-Induced Apoptosis in U251 Glioma Cells

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• 作者：Ya-Ping Liu ; Fu-Xing Dong ; Xiang Chai ; Shuang Zhu…
• 刊名：Cellular and Molecular Neurobiology
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：36
• 期：5
• 页码：737-743
• 全文大小：1,072 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Neurosciences
  Animal Anatomy, Morphology and Histology
  
• 出版者：Springer Netherlands
• ISSN：1573-6830
• 卷排序：36

文摘

In recent years, the role of capsaicin in cancer prevention and treatment has gained people’s attention. However, the mechanism of anti-glioma cells by capsaicin has not been elucidated. Here, we discuss the mechanism of capsaicin in U251 cells. Cell viability was detected by MTT and extracellular LDH measurements, while immunofluorescence was performed to measure changes of LC3 in U251 cells. The expressions of LC3II, Puma-α, Beclin1, P62, Procaspase-3, and P53 were observed by immunoblotting. The cell viability decreased and the punctate patterns of LC3 in U251 cells were observed after Capsaicin treatment. Meanwhile, the expressions of Beclin1, P62, and Puma-α increased. After using 3-MA, the expressions of Beclin1 and Procaspase-3 were reduced while those of P53 and Puma-α increased. The expression of LC3II was increased after Pifithrin-α treatment. Therefore, we believed that capsaicin could induce apoptosis in U251 cells, and the inhibition of autophagy could contribute to apoptosis.KeywordsCapsaicinAutophagyGliomaApoptosis