An injectable hydrogel with or without drugs for prevention of epidural scar adhesion after laminectomy in rats

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• 作者：Lin Chen ; Xiang-qian Li ; Lu-ping Cao ; Xi-lei Li…
• 关键词：Thermo ; reversible hydrogel ; Epidural scarring ; Laminectomy ; Steroid hormone
• 刊名：Chinese Journal of Polymer Science
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：34
• 期：2
• 页码：147-163
• 全文大小：2,590 KB
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• 作者单位：Lin Chen (1)
  Xiang-qian Li (2)
  Lu-ping Cao (1)
  Xi-lei Li (1) (2)
  Jun-rong Meng (2)
  Jian Dong 钁ｅ仴 (2)
  Lin Yu 淇為簾 (1)
  Jian-dong Ding (1)
  
  1. State Key Laboratory of Molecular Engineering of Polymers & Department of Macromolecular Science, Fudan University, Shanghai, 200433, China
  2. Department of Orthopaedic Surgery, Zhongshan Hospital, Fudan University, Shanghai, 200032, China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Condensed Matter Physics
  Industrial Chemistry and Chemical Engineering
  Polymer Sciences
  Characterization and Evaluation of Materials
  
• 出版者：Chinese Chemical Society and Institute of Chemistry, CAS, co-published with Springer
• ISSN：1439-6203

文摘

Epidural scarring occurs inevitably in the defect after spinal laminectomy, and thus how to prevent or reduce it becomes a challenging topic. In the present study, an injectable hydrogel and its dexamethasone (DEX)-loaded hydrogel systems were adopted to prevent epidural scarring in a postlaminectomy rat model. The hydrogel system composed of poly(D,L-lactic acid-co-glycolic acid)-poly(ethylene glycol)-poly(D,L-lactic acid-co-glycolic acid) (PLGA-PEG-PLGA) triblock copolymers was a free-flowing sol at room temperature, and spontaneously turned into a semi-solid gel at body temperature. A lumbar 3 total laminectomy was performed on Sprague Dawley (SD) rats, and the efficacy of the injectable hydrogel with or without drugs in preventing epidural scar formation was evaluated via the gross anatomical observation and histological examination at one month post-surgery. The results demonstrate that the use of hydrogel alone reduced epidural scarring significantly, whereas the efficacy of the DEX-loaded hydrogels presented an irregular dose-dependency of drug and even the inappropriate drug doses resulted in the negative results. Therefore, the present study confirms that the PLGA-PEGPLGA hydrogel holds potential as a barrier device to decrease peridural scarring, and reveals that the sustained delivery of the steroid hormone DEX to prevent surgery-related adhesions in the laminectomy defect is complex. Moreover, our in vivo studies also remind the researchers to pay attention to the irregular dose-dependency of the hormone drugs. Keywords Thermo-reversible hydrogel Epidural scarring Laminectomy Steroid hormone