Overexpression of a Xylanase Inhibitor Gene, OsHI-XIP, Enhances Resistance in Rice to Herbivores

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• 作者：Zhaojun Xin (1)
  Qi Wang (1)
  Zhaonan Yu (1)
  Liucheng Hu (1)
  Jiancai Li (1)
  Caiyu Xiang (1)
  Baohui Wang (1)
  Yonggen Lou (1)
  
• 关键词：Rice ; Xylanase inhibitor ; Chilo suppressalis ; Nilaparvata lugens ; Herbivore ; induced defense response
• 刊名：Plant Molecular Biology Reporter
• 出版年：2014
• 出版时间：April 2014
• 年：2014
• 卷：32
• 期：2
• 页码：465-475
• 全文大小：864 KB
• 作者单位：Zhaojun Xin (1)
  Qi Wang (1)
  Zhaonan Yu (1)
  Liucheng Hu (1)
  Jiancai Li (1)
  Caiyu Xiang (1)
  Baohui Wang (1)
  Yonggen Lou (1)
  
  1. State Key Laboratory of Rice Biology, Institute of Insect Science, Zhejiang University, Hangzhou, 310058, China
  
• ISSN：1572-9818

文摘

Xylanase inhibitors have been reported to play an important role in plant defense against fungal pathogens. However, little to nothing is known about their role in defense against herbivores. Here, we cloned a rice xylanase-inhibiting protein (XIP)-type gene OsHI-XIP whose encoding protein is located in the endoplasmic reticulum (ER). Transcriptional analysis revealed a low constitutive mRNA level of OsHI-XIP, while herbivore infestation, mechanical wounding, and treatment with jasmonic acid (JA), a signal that can be induced after infestation by the rice striped stem borer (SSB) Chilo suppressalis, result in an obvious increase in transcript levels. Overexpression of OsHI-XIP, which increased mRNA levels of the gene by 14.7- to 17.7-fold and 7.5- to 9.6-fold at 0 and 12?h, respectively, after SSB infestation, enhanced constitutive and SSB-induced xylanase inhibitor activity and reduced the larval performance of SSB. Moreover, overexpression of OsHI-XIP decreased the feeding and oviposion preferences of the rice brown planthopper (BPH) Nilaparvata lugens, but did not influence the growth and development of rice plants. The results suggest that the rice XIP-type xylanase inhibitor OsHI-XIP is involved in the resistance in rice to herbivores.