Culture of newborn monkey liver epithelial progenitor cells in chemical defined serum-free medium

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• 作者：Shaohui Ji (1) (3) (4)
  Lifang Jin (1) (2)
  Xiangyu Guo (1) (3) (4)
  Weizhi Ji (1) (3) (5)
  
• 关键词：Hepatic progenitor cells ; Bipotency ; Serum free ; Extracelullar matrix ; Rhesus monkey
• 刊名：In Vitro Cellular & Developmental Biology - Animal
• 出版年：2010
• 出版时间：September 2010
• 年：2010
• 卷：46
• 期：8
• 页码：693-701
• 全文大小：546KB
• 参考文献：1. Arends B.; Spee B.; Schotanus B. A.; Roskams T.; van den Ingh T. S.; Penning L. C.; Rothuizen J. In vitro differentiation of liver progenitor cells derived from healthy dog livers. / Stem Cells Dev 18: 351-58; 2009. CrossRef
  2. Azuma H.; Hirose T.; Fujii H.; Oe S.; Yasuchika K.; Fujikawa T.; Yamaoka Y. Enrichment of hepatic progenitor cells from adult mouse liver. / Hepatology 37: 1385-394; 2003. CrossRef
  3. Brill S.; Zvibel I.; Reid L. M. Expansion conditions for early hepatic progenitor cells from embryonal and neonatal rat livers. / Dig Dis Sci 44: 364-71; 1999. CrossRef
  4. Chen Q.; Kon J.; Ooe H.; Sasaki K.; Mitaka T. Selective proliferation of rat hepatocyte progenitor cells in serum-free culture. / Nat Protoc 2: 1197-205; 2007. CrossRef
  5. Dunn J. C.; Tompkins R. G.; Yarmush M. L. Long-term in vitro function of adult hepatocytes in a collagen sandwich configuration. / Biotechnol Prog 7: 237-45; 1991. CrossRef
  6. Elsdale T.; Bard J. Collagen substrata for studies on cell behavior. / J Cell Biol 54: 626-37; 1972. CrossRef
  7. Factor V. M.; Radaeva S. A.; Thorgeirsson S. S. Origin and fate of oval cells in dipin-induced hepatocarcinogenesis in the mouse. / Am J Pathol 145: 409-22; 1994.
  8. Herrera M. B.; Bruno S.; Buttiglieri S.; Tetta C.; Gatti S.; Deregibus M. C.; Bussolati B.; Camussi G. Isolation and characterization of a stem cell population from adult human liver. / Stem Cells 24: 2840-850; 2006. CrossRef
  9. Hoppo T.; Fujii H.; Hirose T.; Yasuchika K.; Azuma H.; Baba S.; Naito M.; Machimoto T.; Ikai I. Thy1-positive mesenchymal cells promote the maturation of CD49f-positive hepatic progenitor cells in the mouse fetal liver. / Hepatology 39: 1362-370; 2004. CrossRef
  10. Jin L.; Ji S.; Tang X.; Guo X.; Lu Y.; Chen H.; Deng H.; Zhou Q.; Ji W. Isolation and characterization of liver epithelial progenitor cells from normal adult rhesus monkeys (Macaca mulatta). / Cell Res 19: 268-70; 2009. CrossRef
  11. Kano J.; Noguchi M.; Kodama M.; Tokiwa T. The in vitro differentiating capacity of nonparenchymal epithelial cells derived from adult porcine livers. / Am J Pathol 156: 2033-043; 2000.
  12. Koebe H. G.; Pahernik S.; Eyer P.; Schildberg F. W. Collagen gel immobilization: a useful cell culture technique for long-term metabolic studies on human hepatocytes. / Xenobiotica 24: 95-07; 1994. CrossRef
  13. Koenig S.; Krause P.; Drabent B.; Schaeffner I.; Christ B.; Schwartz P.; Unthan-Fechner K.; Probst I. The expression of mesenchymal, neural and haematopoietic stem cell markers in adult hepatocytes proliferating in vitro. / J Hepatol 44: 1115-124; 2006a. CrossRef
  14. Koenig S.; Probst I.; Becker H.; Krause P. Zonal hierarchy of differentiation markers and nestin expression during oval cell mediated rat liver regeneration. / Histochem Cell Biol 126: 723-34; 2006b. CrossRef
  15. Malhi H.; Irani A. N.; Gagandeep S.; Gupta S. Isolation of human progenitor liver epithelial cells with extensive replication capacity and differentiation into mature hepatocytes. / J Cell Sci 115: 2679-688; 2002.
  16. Nierhoff D.; Ogawa A.; Oertel M.; Chen Y. Q.; Shafritz D. A. Purification and characterization of mouse fetal liver epithelial cells with high in vivo repopulation capacity. / Hepatology 42: 130-39; 2005. CrossRef
  17. Nitou M.; Sugiyama Y.; Ishikawa K.; Shiojiri N. Purification of fetal mouse hepatoblasts by magnetic beads coated with monoclonal anti-e-cadherin antibodies and their in vitro culture. / Exp Cell Res 279: 330-43; 2002. CrossRef
  18. Nussler A.; Konig S.; Ott M.; Sokal E.; Christ B.; Thasler W.; Brulport M.; Gabelein G.; Schormann W.; Schulze M.; Ellis E.; Kraemer M.; Nocken F.; Fleig W.; Manns M.; Strom S. C.; Hengstler J. G. Present status and perspectives of cell-based therapies for liver diseases. / J Hepatol 45: 144-59; 2006. CrossRef
  19. Nyamath P.; Alvi A.; Habeeb A.; Khosla S.; Khan A. A.; Habibullah C. M. Characterization of hepatic progenitors from human fetal liver using CD34 as a hepatic progenitor marker. / World J Gastroenterol 13: 2319-323; 2007.
  20. Paku S.; Schnur J.; Nagy P.; Thorgeirsson S. S. Origin and structural evolution of the early proliferating oval cells in rat liver. / Am J Pathol 158: 1313-323; 2001.
  21. Preisegger K. H.; Factor V. M.; Fuchsbichler A.; Stumptner C.; Denk H.; Thorgeirsson S. S. Atypical ductular proliferation and its inhibition by transforming growth factor beta1 in the 3, 5-diethoxycarbonyl-1, 4-dihydrocollidine mouse model for chronic alcoholic liver disease. / Lab Invest 79: 103-09; 1999.
  22. Rao M. S.; Khan A. A.; Parveen N.; Habeeb M. A.; Habibullah C. M.; Pande G. Characterization of hepatic progenitors from human fetal liver during second trimester. / World J Gastroenterol 14: 5730-737; 2008. CrossRef
  23. Sasaki K.; Kon J.; Mizuguchi T.; Chen Q.; Ooe H.; Oshima H.; Hirata K.; Mitaka T. Proliferation of hepatocyte progenitor cells isolated from adult human livers in serum-free medium. / Cell Transplant 17: 1221-230; 2008. CrossRef
  24. Schmelzer E.; Zhang L.; Bruce A.; Wauthier E.; Ludlow J.; Yao H. L.; Moss N.; Melhem A.; McClelland R.; Turner W.; Kulik M.; Sherwood S.; Tallheden T.; Cheng N.; Furth M. E.; Reid L. M. Human hepatic stem cells from fetal and postnatal donors. / J Exp Med 204: 1973-987; 2007. CrossRef
  25. Sun X. Y.; An J. Expression of nestin, an intermediate filament protein, in human fetal hepatic stem cells. / Di Yi Jun Yi Da Xue Xue Bao 24: 207-09; 2004.
  26. Suzuki A.; Zheng Y. W.; Kaneko S.; Onodera M.; Fukao K.; Nakauchi H.; Taniguchi H. Clonal identification and characterization of self-renewing pluripotent stem cells in the developing liver. / J Cell Biol 156: 173-84; 2002. CrossRef
  27. Thiery J. P. Epithelial-mesenchymal transitions in development and pathologies. / Curr Opin Cell Biol 15: 740-46; 2003. CrossRef
  28. Tsuchiya A.; Heike T.; Fujino H.; Shiota M.; Umeda K.; Yoshimoto M.; Matsuda Y.; Ichida T.; Aoyagi Y.; Nakahata T. Long-term extensive expansion of mouse hepatic stem/progenitor cells in a novel serum-free culture system. / Gastroenterology 128: 2089-104; 2005. CrossRef
  29. Turner W. S.; Schmelzer E.; McClelland R.; Wauthier E.; Chen W.; Reid L. M. Human hepatoblast phenotype maintained by hyaluronan hydrogels. / J Biomed Mater Res 82: 156-68; 2007. CrossRef
  30. Valdes F.; Alvarez A. M.; Locascio A.; Vega S.; Herrera B.; Fernandez M.; Benito M.; Nieto M. A.; Fabregat I. The epithelial mesenchymal transition confers resistance to the apoptotic effects of transforming growth factor beta in fetal rat hepatocytes. / Mol Cancer Res 1: 68-8; 2002.
  31. Wiese C.; Rolletschek A.; Kania G.; Blyszczuk P.; Tarasov K. V.; Tarasova Y.; Wersto R. P.; Boheler K. R.; Wobus A. M. Nestin expression—a property of multi-lineage progenitor cells? / Cell Mol Life Sci 61: 2510-522; 2004. CrossRef
  32. Zhang W.; Chen X. P.; Zhang W. G.; Zhang F.; Xiang S.; Dong H. H.; Zhang L. Hepatic non-parenchymal cells and extracellular matrix participate in oval cell-mediated liver regeneration. / World J Gastroenterol 15: 552-60; 2009. CrossRef
  
• 作者单位：Shaohui Ji (1) (3) (4)
  Lifang Jin (1) (2)
  Xiangyu Guo (1) (3) (4)
  Weizhi Ji (1) (3) (5)
  
  1. Kunming Primate Research Center, and Kunming Institute of Zoology, Chinese Academy of Sciences, 32# Jiaochang Donglu, Kunming, Yunnan, 650223, China
  3. Yunnan Key Laboratory of Animal Reproductive Biology, Kunming, Yunnan, China
  4. Graduate School, Chinese Academy of Sciences, Beijing, China
  2. College of Life Science, Shaoxing University, 900# Chennan Dadao, Shaoxing, Zhejiang, 312000, China
  5. Kunming Biomed International, Kunming, Yunnan, China
  

文摘

Studies with hepatic progenitor cells from non-human primates would allow better understanding of their human counterparts. In this study, rhesus monkey liver epithelial progenitor cells (mLEPCs) were derived from a small piece of newborn livers in chemical defined serum-free medium. Digested hepatic cells were treated in Ca2+-containing medium to form cell aggregates. Two types of cell aggregates were generated: elongated spindle cells and polygonal epithelial cells. Elongated spindle cells were expressed as vimentin and brachyury, and they were disappeared within 5?d in our cultures. The remaining type consisted of small polygonal epithelial cells that expressed cytokeratin 7 (CK7), CK8, CK18, nestin, CD49f, and E-cad, the markers of hepatic stem cells, but were negative for α-fetoprotein, albumin, and CK19. They can proliferate and be passaged, if on laminin or rat tail collagen gel, to initiate colonies. When cultured with dexamethasone and oncostatin M, the expression of mature hepatocyte markers, such as α-1-antitrypsin, intracytoplasmic glycogen storage, indocyanine green uptake, and lipid droplet generation, were induced in differentiated cells. If transferred onto mouse embryonic fibroblasts feeders, they gave rise to CK19-positive cholangiocytes with formation of doughnut-like structure. Thus, mLEPCs with bipotency were derived from newborn monkey liver and may serve as a preclinical model for assessment of cell therapy in humans.