Preparation and characterization of cationic PLGA-PEGLf/DOPE nanoparticles for HO-1 gene delivery

详细信息    查看全文

• 作者：Yuanjian Song (12) biosongyuanjian@126.com
  Zhimin Zong (1)
  Hongzhi Liu (2)
  Xiangru Wen (2)
  Dashi Qi (2)
  Yang Du (2)
  Fang Zhang (2)
  Xianyong Wei (1) wei_xianyong@163.com
• 关键词：Key words PLGA nanoparticles – ; PEGylation – ; gene delivery – ; lactoferrin
• 刊名：Journal of Wuhan University of Technology--Materials Science Edition
• 出版年：2012
• 出版时间：April 2012
• 年：2012
• 卷：27
• 期：2
• 页码：217-221
• 全文大小：419.3 KB
• 参考文献：1. Mulligan R C. The Basic Science of Gene Therapy[J]. Science, 1993, 260: 926–931
  2. Li SD, Huang L. Non-viral is Superior to Viral Gene Delivery[J]. J. Controlled Release, 2007, 123(3): 181–183
  3. Wolfert M A, Schact E H, Toneheva V, et a1. Characterization of Vectors for Gene Therapy Formed by Self-assembly of DNA with Synthetic Block Copolymers[J]. Hum. Gene Ther., 1996, 7(4): 2 123–2 128
  4. Zaumer W, Brunner S, Busehle M, et a1. Diferential Behavior of Lipid Based and Polycation Based Gene Transfer Systems in Transfecting Primary Human Fibroblasts: A Potential Role of Polylysine in Nuclear Transport[J]. Biochim. Biophys. Acta, 1999, 1428(1): 57–61
  5. Fischera D, Li YX, Ahlemeyerc B, et a1. In vitro Cytotoxicity Testing of Polycations: Influence of Polymer Structure on Cell Viability and Hemolysis[J]. Biomaterials, 2003, 24(7): 1 121–1 131
  6. Jeon O, Lim H W, Lee M, et a1. Poly(l-lactide-co-glycolide) Nanospheres Conjugated with a Nuclear Localization Signal for Delivery of Plasmid DNA[J]. J. Drug Target, 2007, 15(3): 190–198
  7. Park Y M, Shin B A, Oh J I, et a1. Poly(L-lactic acid)/Polyethylenimine Nanoparticles as Plasmid DNA Carriers[J]. Arch. Pharm. Res., 2008, 31(1): 96–102
  8. Guo XD, Song YL, Zheng QX, et a1. Surface Modification of Biomimetic PLGA-(ASP-PEG) Matrix with RGD-containing Peptide: A New Non-viral Vector for Gene Transfer and Tissue Engineering[J]. J. Wuhan University of Tech.-Mate. Sci. Ed., 2006, 21(3): 41–43
  9. Pan HT, Zheng QX, Guo XD. TGF-beta 1 Gene-activated Matrices Regulated the Osteogenic Differentiation of BMSCs[J]. J. Wuhan University of Tech-Mate. Sci. E., 2007, 22(3): 431–435
  10. Luten J, Van Nostrum C F, De Smedt S C, et a1. Biodegradable Polymers as Non-viral Carriers for Plasmid DNA Delivery[J]. J. Controlled Release, 2008, 126(2): 97–110
  11. Hao J, Hu ZM, Zheng QX, et a1. Biomineralization of the Surface of PLGA-(ASP-PEG) Modified with the K16 and RGD-containing Peptide[J]. J. Wuhan University of Tech.-Mate. Sci. Ed., 2009, 24(5): 768–771
  12. Mao SR, Shuai XT, Unger F, et a1. Synthesis, Characterization and Cytotoxicity of Poly(ethylene glycol)-graft-trimethyl Chitosan Block Copolymers[J]. Biomaterials, 2005, 26(32): 6 343–6 356
  13. Huang RQ, Qu YH, Ke WL, et a1. Efficient Gene Delivery Targeted to the Brain Using a Transferrin-conjugated Polyethyleneglycol-modified Polyamidoamine Dendrimer[J]. FASEB J., 2007, 21: 1117–1125
  14. Singh M, Fang JH, Kazzaz J, et a1. A Modified Process for Preparing Cationic Polylactide-co-glycolide Microparticles with Adsorbed DNA[J]. Int. J. Pharm., 2006, 327(1–2): 1–5
  15. Basarkar A, Devineni D, Palaniappan R, et a1. Preparation, Characterization, Cytotoxicity and Transfection Efficiency of Poly(dl-Lactide-co-glycolide) and Poly(dl-lactic acid) Cationic Nanoparticles for Controlled Delivery of Plasmid DNA[J]. J. Singh. Int. J. Pharm., 2007, 343(1–2): 247–254
  16. Liang XF, Wang HJ, Luo H, et a1. Preparation and Characterization of Novel Biodegradable Multifunctional Microspheres[J]. Acta Chim. Sinica, 2008, 66(19): 2 178–2 183
  17. Liang XF, Tian H, Luo H, et a1. Novel Quaternized Chitosan and Polymeric Micelles with Cross-linked Ionic Cores for Prolonged Release of Minocycline[J]. J. Biomater. Sci., Polym. Ed., 2009, 20(1): 115–131
  18. Lu W, Zhang Y, Tan YZ, et a1. Cationic Albumin-conjugated Pegylated Nanoparticles as Novel Drug Carrier for Brain Delivery[J]. J. Controlled Release, 2005, 107(3): 428–448
  19. Ellmann G L. Tissue Sulfhydryl Groups[J]. Arch. Biochem. Biophys., 1959, 82(1): 70–77
  20. Liang XF, Wang HJ, Luo H, et a1. Characterization of Novel Multifunctional Cationic Polymeric Liposomes Formed from Octadecyl Quaternized Carboxymethyl Chitosan/cholesterol and Drug Encapsulation[J]. Langmuir, 2008, 24(14): 7 147–7 153
  21. Choi S H, Jin S E, Lee M K, et a1. Novel Cationic Solid Lipid Nanoparticles Enhanced p53 Gene Rransfer to Lung Cancer Cells[J]. Eur. J. Pharm. Biopharm., 2008, 68(3): 545–554
• 作者单位：1. Key Laboratory of Coal Processing and Efficient Utilization (Ministry of Education), China University of Mining & Technology, Xuzhou, 221008 China2. Department of Neurobiology, Xuzhou Medical College, Xuzhou, 221002 China
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Materials Science
  Chinese Library of Science
  
• 出版者：Wuhan University, co-published with Springer
• ISSN：1993-0437

文摘

Cationic nanoparticles (NPs) for gene delivery were successfully prepared by assembling carboxylation poly(lactic-co-glycolic acid) (PLGA), polyethylene glycol (PEG), L-α-Phosphatidylethanolamine (DOPE) and octadecyl quaternized carboxymethyl chitosans (OQCMC). Lactoferrin (Lf) was selected as a targeting ligand conjugated to PLGA via bifunctional PEG, yielding PLGA-PEG-Lf/DOPE NPs to be used for gene vectors. Fourier transform infrared spectroscopy (FTIR), UV and nuclear magnetic resonance (NMR) spectroscopy were performed to evaluate the synthesis of the vectors. The characteristics of the vectors loaded heme oxygenase (HO-1) gene were evaluated by transmission electron microscope (TEM), particle size analyser and fluorescent microscopy. The experimental results showed that the obtained vectors were spherical in shape with average particle size of 142.2 nm and zeta potentials of +16.4 mV. The vectors could protect the loaded gene from the degradation by nuclease. For 293T cells, there is high transfection efficiency of the vectors similar to liposome-2000. It can be concluded that the established cationic PLGA-PEG-Lf/DOPE NPs have potential gene delivery ability for gene therapy.