Development of 39 novel polymorphic microsatellite markers for the giant mottled eel Anguilla marmorata and cross-amplification in other eel species

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• 作者：Xiaolu Wang (1) (3)
  Fang Xu (2) (3)
  Li Li (1) (3)
  Yaping Zhang (1) (3)
  Yandong Ding (1) (3)
  Xue Zang (1) (3)
  Yihe Jia (1) (3)
  Guosong Zhang (1) (3)
  Yayuan Wang (1) (3)
  Xiaojun Wang (1) (3)
  Hongwei Zhang (1) (3)
  Shaowu Yin (1) (3)
  
• 关键词：Anguilla marmorata ; Microsatellite markers ; Cross ; amplification
• 刊名：Conservation Genetics Resources
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：6
• 期：4
• 页码：865-871
• 全文大小：173 KB
• 参考文献：1. Gao ZX, Luo W, Liu H, Zeng C, Liu XL, Yi SK, Wang WM (2012) Transcriptome analysis and SSR/SNP markers information of the Blunt Snout Bream ( / Megalobrama amblycephala). PLoS ONE 7:1-0 CrossRef
  2. Gong XL, Ren SJ, Chen SQ, Jia ZY (2009) Highly polymorphic microsatellite loci from the giant mottled eel ( / Anguilla marmorata). Mol Ecol Resour 9:1544-547 CrossRef
  3. Kazuharu N, Kagayaki M, Hideki T, Tatsuya U, Koichi O, Hiromi O, Katsutoshi A (2006) Microsatellite-centromere mapping in the Japanese eel ( / Anguilla japonica) by half-tetrad analysis using induced triploid families. Aquaculture 257:53-7 CrossRef
  4. Schuelke M (2000) An economic method for the use of fluorescent labeling of PCR fragments. Nat Biotech 18:233-34 CrossRef
  5. Tseng MC (2012) Evolution of microsatellite loci of tropical and temperate anguilla eels. Int J Mol Sci 13:4281-294 CrossRef
  
• 作者单位：Xiaolu Wang (1) (3)
  Fang Xu (2) (3)
  Li Li (1) (3)
  Yaping Zhang (1) (3)
  Yandong Ding (1) (3)
  Xue Zang (1) (3)
  Yihe Jia (1) (3)
  Guosong Zhang (1) (3)
  Yayuan Wang (1) (3)
  Xiaojun Wang (1) (3)
  Hongwei Zhang (1) (3)
  Shaowu Yin (1) (3)
  
  1. Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, 210023, China
  3. Co-Innovation Center for Marine Bio-Industry Technology, Lian Yungang, 222005, China
  2. School of Businesss, Nanjing Normal University, Nanjing, 210023, China
  
• ISSN：1877-7260

文摘

In this study, we have developed microsatellite markers for the giant mottled eel (Anguilla marmorata) from expressed sequence tag (EST) sequences using next-generation sequencing. Consequently, a total of 39 novel polymorphic microsatellite markers were identified in 32 individuals from Wangquan River wild population area. The range of observed heterozygosity, expected heterozygosity and polymorphism information content were 0.044-.000, 0.285-.946 and 0.3783-.9016, respectively. In addition, eight loci were deviated from Hardy–Weinberg equilibrium and modified by Bonferroni correction, and the results showed no significant linkage disequilibrium between the locus pair. The amplification of cross-species was conducted in Anguilla bicolor pacifica and Anguilla japonica, which revealed the applicability of EST-SSR primers. These EST-SSR markers can provide sufficient polymorphism for population genetic studies, pedigree and genome mapping of these three kinds of closely related eels.