Fluorescence dynamics of N-terminal Trp–Trp residues in polypeptide: intrinsic indicators for monitoring pH
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- 作者：Lei Li ; Hua Yi ; Mengfang Chang ; Xiaodan Cao ; Zhongneng Zhou…
- 关键词：Trp–Trp dipeptide ; Fluorescence ; Intrinsic pH sensors
- 刊名：Chinese Science Bulletin
- 出版年：2015
- 出版时间：December 2015
- 年：2015
- 卷：60
- 期：24
- 页码：2129-2134
- 全文大小：1,141 KB
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Hua Yi (1)
Mengfang Chang (1)
Xiaodan Cao (1)
Zhongneng Zhou (1)
Cuifang Qin (1)
Sanjun Zhang (1)
Haifeng Pan (1)
Yan Chen (2)
Jianhua Xu (1)
1. State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai, 200062, China
2. Tongji Hospital Affiliated to Tongji University, Shanghai, 200065, China - 刊物主题：Science, general; Life Sciences, general; Physics, general; Chemistry/Food Science, general; Earth Sciences, general; Engineering, general;
- 出版者：Springer Berlin Heidelberg
- ISSN：1861-9541
文摘
pH plays a vital role in various cellular activities, and real-time observation of the intracellular pH through a pH indicator is very important for studying many physiological processes. In this paper, we studied the pH response of Trp–Trp dipeptide and its derivatives (NATrp2Me, NBTrp2 and Trp2Me) by steady-state and time-resolved fluorescence spectroscopy. Both the fluorescence intensities and lifetimes of Trp–Trp dipeptide as well as Trp2Me were functions of pH in the physiological range from 5.5 to 9.0. However, NATrp2Me and NBTrp2 showed no difference. The exposed amino was found to be pivotal for its pH dependence. Moreover, an artificially synthesized tetrapeptide (Trp–Trp–Ala–Ser) confirmed the pH sensitivity of N-terminal Trp–Trp residues. The pH values could be quantitatively determined from the fluorescence intensities and lifetimes of the N-terminal Trp–Trp residue. Thus, the N-terminal Trp–Trp residues may be fused into the polypeptides/proteins to serve as an intrinsic pH indicator in fluorescence spectroscopy and imaging. Keywords Trp–Trp dipeptide Fluorescence Intrinsic pH sensors