In vivo histological evaluation of fractional ablative microplasma radio frequency technology using a roller tip: an animal study

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• 作者：Xiaodan Li ; Lin Fang ; Luping Huang
• 关键词：Microplasma radio frequency ; Fractional skin resurfacing ; Collagen ; Heat shock protein
• 刊名：Lasers in Medical Science
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：30
• 期：9
• 页码：2287-2294
• 全文大小：1,871 KB
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• 作者单位：Xiaodan Li (1)
  Lin Fang (1)
  Luping Huang (1)
  
  1. Cosmetic Laser Center, Plastic Surgery Hospital of Chinese Academic of Medical Science, Peking Union Medical College, No. 33 Ba-Da-Chu Road, Shijingshan District, Beijing, China
  
• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Medicine/Public Health, general
  Dentistry
  Laser Technology and Physics and Photonics
  Quantum Optics, Quantum Electronics and Nonlinear Optics
  Applied Optics, Optoelectronics and Optical Devices
  
• 出版者：Springer London
• ISSN：1435-604X

文摘

The aim of the present study is to investigate the histological characteristics associated with microplasma radio frequency (MPRF) technology in an animal study using different treatment parameters. Two white piglets, aged 6 months, received MPRF treatment using a roller tip; the treatment site was located on the dorsal skin. Four groups of parameters were adopted regarding the performance of the treatment at four zones on the dorsum. Immediately, at 7 days and at 1, 3, and 6 months posttreatment, we observed the healing process and obtained specimens from each treatment zone. Hematoxylin and eosin and Masson stainings of histological sections were performed to assess the degree of tissue injury, the heat effect, the healing process, and neocollagenesis. Heat shock protein (HSP) was also detected using immunohistochemistry. The roller tip generated a fractional treatment, which had a general trend involving an increase in depth and width with increasing pulse energy and decreasing sliding speed. During the wound healing process, dermal neocollagenesis was stimulated, remodeled, and matured gradually. The expression of HSP47 and HPS72 was elevated in the dermis surrounding the microlesions after treatment; it peaked at 1 month posttreatment and became diffuse in the dermis. MPRF is a promising fractional skin resurfacing technique. The roller tip can be used with low risk in the entire treatment zone with rapid healing. An appropriate treatment regimen should be chosen to guarantee therapeutic efficacy and safety. Keywords Microplasma radio frequency Fractional skin resurfacing Collagen Heat shock protein