Electrochemical aptasensor for lysozyme based on a gold electrode modified with a nanocomposite consisting of reduced graphene oxide, cuprous oxide, and plasma-polymerized propargylamine

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• 作者：Shaoming Fang ; Xiaodong Dong ; Hongfei Ji ; Shunli Liu ; Fufeng Yan…
• 关键词：Plasma polymerization ; Differential pulse voltammetry ; Electrochemical impedance spectroscopy ; Aptasensor ; Hexacyanoferrate ; Field emission scanning electron microscopy ; FTIR
• 刊名：Microchimica Acta
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：183
• 期：2
• 页码：633-642
• 全文大小：796 KB
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• 作者单位：Shaoming Fang (1) (2)
  Xiaodong Dong (1)
  Hongfei Ji (1)
  Shunli Liu (1)
  Fufeng Yan (1)
  Donglai Peng (1)
  Linghao He (1)
  Minghua Wang (1)
  Zhihong Zhang (1) (2)
  
  1. Henan Provincial Key Laboratory of Surface and Interface Science, Zhengzhou University of Light Industry, No. 166, Science Avenue, Zhengzhou, 450001, China
  2. Henan Collaborative Innovation Center of Environmental Pollution Control and Ecological Restoration, Zhengzhou University of Light Industry, No. 166, Science Avenue, Zhengzhou, 450001, China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Inorganic Chemistry
  Physical Chemistry
  Characterization and Evaluation Materials
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  
• 出版者：Springer Wien
• ISSN：1436-5073

文摘

This article describes an aptasensor for lysozyme that is based on a gold electrode modified with an aptamer-wrapped composite consisting of three-dimensional reduced graphene oxide, cuprous oxide, and plasma-polymerized propargylamine (Cu<sub>2sub>O@rGO@PpPG) as the sensing layer. The nanocomposite consisting of Cu<sub>2sub>O@rGO was synthesized by simultaneously reducing GO and Cu(II) ions with glucose and then modified by plasma-enhanced chemical vapor deposition using propargylamine as the monomer gas. The resulting amine-rich nanofilms of Cu<sub>2sub>O@rGO@PpPG nanocomposite were deposited on a gold electrode. Differential pulse voltammetry and electrochemical impedance spectroscopy show these layers to exhibit a good amperometric response and variation of the charge transfer resistance to lysozyme after aptamer strands had been immobilized on the films via electrostatic interaction between the negatively charged phosphate groups of the aptamer and the positively charged amino groups on the electrode. The sensor, when operated at 0.22 V (vs. Ag/AgCl), can detect lysozyme in the 0.1 nM to 200 nM concentration range with a 0.06 nM limit of detection. In addition, the sensor displays excellent selectivity and repeatability. In our perception, this strategy for preparing aptasensors holds a great potential with respect to the use of plasma-modified nanocomposites in clinical analysis.