Comparative Diversity Analysis of Gut Microbiota in Two Different Human Flora-Associated Mouse Strains
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- 作者：Xiaojing Zhang (1)
Benhua Zeng (2)
Zhiwei Liu (1)
Zhenlin Liao (1)
Wenxai Li (2)
Hong Wei (2)
Xiang Fang (1) - 关键词：Human flora ; associated mice ; C57BL/6J ; KM ; DGGE ; Gut microbiota
- 刊名：Current Microbiology
- 出版年：2014
- 出版时间：September 2014
- 年：2014
- 卷：69
- 期：3
- 页码：365-373
- 全文大小：1,830 KB
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Benhua Zeng (2)
Zhiwei Liu (1)
Zhenlin Liao (1)
Wenxai Li (2)
Hong Wei (2)
Xiang Fang (1)
1. College of Food Science, South China Agricultural University, Guangzhou, 510642, China
2. Department of Laboratory Animal Science, College of Basic Medicine Science, Third Military Medical University, Chongqing, 400038, China - ISSN：1432-0991
文摘
The Kunming (KM) mouse is a closed colony mouse strain widely used in Chinese pharmacology, toxicology, and microbiology research laboratories. However, few studies have examined human flora-associated (HFA) microbial communities in KM mice. In this study, HFA models were built from germ-free KM and C57BL/6J mouse strains, and gut microbial diversity was analyzed by denaturing gradient gel electrophoresis (DGGE) and DNA sequencing. We found that the two strains of HFA mice were significantly different based on the UPGMA dendrogram and the Richness index, but dice similarity coefficients of mouse replicates were not significantly different between HFA-KM and HFA-C57BL/6J. Most of the dominant phyla of human gut microflora could be transferred into the guts of the two mouse strains. However, the predominant genus that formed in HFA-KM was Clostridium sp. and that in HFA-C57BL/6J was Blautia sp. These results imply that genotypes difference between the two mice strains is a critical factor in shaping the intestinal microflora. However, genetic differences of individuals within KM mouse populations failed to lead to individual difference in microflora. Successful generation of HFA-KM mice will facilitate studies examining how diet affects gut microbial structure, and will enable comparative studies for uncovering genetic factors that shape gut microbial communities.