Enolase of Streptococcus Suis Serotype 2 Enhances Blood–Brain Barrier Permeability by Inducing IL-8 Release

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• 作者：Yingying Sun ; Na Li ; Jing Zhang ; Hongtao Liu ; Jianfang Liu ; Xiaojing Xia…
• 刊名：Inflammation
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：39
• 期：2
• 页码：718-726
• 全文大小：1,275 KB
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• 作者单位：Yingying Sun (1)
  Na Li (1)
  Jing Zhang (2)
  Hongtao Liu (1)
  Jianfang Liu (1)
  Xiaojing Xia (1)
  Changjiang Sun (1)
  Xin Feng (1)
  Jingmin Gu (1)
  Chongtao Du (1)
  Wenyu Han (1)
  Liancheng Lei (1)
  
  1. College of Veterinary Medicine, Jilin University, Xi’an Road 5333, Changchun, 130062, China
  2. Changchun University of Chinese Medicine, Changchun, People’s Republic of China
  
• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Rheumatology
  Internal Medicine
  Pharmacology and Toxicology
  Pathology
  
• 出版者：Springer Netherlands
• ISSN：1573-2576

文摘

Streptococcus suis serotype 2 (SS2) is an emerging zoonosis, and meningitis is the most frequent clinical manifestation, but mechanism of its virulent factor, enolase (Eno), is unknown in meningitis. In this study, Eno was inducibly expressed and added to an in vitro Transwell co-culture model of the blood–brain barrier (BBB) consisted of porcine brain microvascular endothelial cells (PBMECs) and astrocytes (ACs), the results showed that Eno induces a significant increase in BBB permeability and promotes the release of IL-8 et al. cytokines. Furthermore, IL-8 could significantly destroy the integrity of the BBB model in vitro. In mice models administered Eno for 24 h, Eno could significantly promote Evans blue (EB) moving from the blood to the brain and significantly increased the serum and brain levels of IL-8, as detected by ELISA. While G31P (IL-8 receptor antagonist) significantly decreased the concentration of EB in the brains of mice injected with Eno. The present study demonstrated that SS2 Eno may play an important role in disrupting BBB integrity by prompting IL-8 release.