MoGrr1, a novel F-box protein, is involved in conidiogenesis and cell wall integrity and is critical for the full virulence of Magnaporthe oryzae

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• 作者：Min Guo ; Fei Gao ; Xiaolei Zhu ; Xiang Nie…
• 关键词：Magnaporthe oryzae ; Conidiation ; Appressoria formation ; Cell wall integrity ; Pathogenicity
• 刊名：Applied Microbiology and Biotechnology
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：99
• 期：19
• 页码：8075-8088
• 全文大小：11,839 KB
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• 作者单位：Min Guo (1)
  Fei Gao (1)
  Xiaolei Zhu (1)
  Xiang Nie (1)
  YueMin Pan (1)
  Zhimou Gao (1)
  
  1. Department of Plant Pathology, College of Plant Protection, Anhui Agricultural University, Hefei, 230036, China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Microbiology
  Microbial Genetics and Genomics
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-0614

文摘

The production of asexual spores plays a critical role in rice blast disease. However, the mechanisms of the genes involved in the conidiogenesis pathway are not well understood. F-box proteins are specific adaptors to E3 ubiquitin ligases that determine the fate of different substrates in ubiquitin-mediated protein degradation and play diverse roles in fungal growth regulation. Here, we identify a Saccharomyces cerevisiae Grr1 homolog, MoGrr1, in Magnaporthe oryzae. Targeted disruption of Mogrr1 resulted in defects in vegetative growth, melanin pigmentation, conidial production, and resistance to oxidative stress, and these mutants consequently exhibited attenuated virulence to host plants. Microscopy studies revealed that the inability to form conidiophores is responsible for the defect in conidiation. Although the Mogrr1 mutants could develop melanized appressoria from hyphal tips, the appressoria were unable to penetrate into plant tissues due to insufficient turgor pressure within the appressorium, thereby attenuating the virulence of the mutants. Quantitative RT-PCR results revealed significantly decreased expression of chitin synthase-encoding genes, which are involved in fungal cell wall integrity, in the Mogrr1 mutants. The Mogrr1 mutants also displayed reduced expression of central components of the MAP kinase and cAMP signaling pathways, which are required for appressorium differentiation. Furthermore, domain complementation analysis indicated that two putative protein-interacting domains in MoGrr1 play essential roles during fungal development and pathogenicity. Taken together, our results suggest that MoGrr1 plays essential roles in fungal development and is required for the full virulence of M. oryzae. Keywords Magnaporthe oryzae Conidiation Appressoria formation Cell wall integrity Pathogenicity