Silencing GTSE-1 expression inhibits proliferation and invasion of hepatocellular carcinoma cells

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• 作者：Lei Guo ; Shumin Zhang ; Bo Zhang ; Wanyong Chen ; Xiaoqiang Li…
• 刊名：Cell Biology and Toxicology
• 出版年：2016
• 出版时间：August 2016
• 年：2016
• 卷：32
• 期：4
• 页码：263-274
• 全文大小：6,759 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Cell Biology
  Pharmacology and Toxicology
  Biochemistry
  
• 出版者：Springer Netherlands
• ISSN：1573-6822
• 卷排序：32

文摘

G2 and S phase-expressed-1 (GTSE1) was recently reported to upregulate in several types of human cancer, based on negatively regulate p53 expression. However, its expression and functional roles in hepatocellular carcinoma (HCC) remain unknown. In this study, GTSE1 was observed to be highly expressed in HCC specimens and cell lines both at messenger RNA (mRNA) and protein levels. Furthermore, high GTSE1 expression was positively associated with tumor size, venous invasion, advanced tumor stage, and short overall survival. Moreover, we generated stable GTSE1 knockdown HCC cell lines to explore the effects of GTSE1 silencing on the growth and invasion of HCC in vitro. In determining the pathway through which GTSE1 regulated cell proliferation and invasion, GTSE1 silencing was found to inhibit AKT phosphorylation and downregulated cell cycle-related protein. In addition, GTSE1 downregulation decreased the growth of xenografts. In conclusion, these results indicated for the first time that overexpression of GTSE1 was involved in the progress of HCC, enhancing proliferation and promoting cell invasion in HCC cells.KeywordsHCCGTSE1ProliferationInvasionPrognosis