Establishment and partial characterization of a human tumor cell line, GBM-HSF, from a glioblastoma multiforme

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• 作者：Jiagui Qu (1) (2)
  Joshua D. Rizak (2) (3)
  Yaodong Fan (4)
  Xiaoxuan Guo (5)
  Jiejing Li (6)
  Tanzeel Huma (2) (7)
  Yuanye Ma (2)
  
• 关键词：Cell line ; Glioblastoma multiforme ; Left prefrontal lobe ; Gene transcription
• 刊名：Human Cell
• 出版年：2014
• 出版时间：July 2014
• 年：2014
• 卷：27
• 期：3
• 页码：129-136
• 全文大小：3,842 KB
• 参考文献：1. Van Meir EG, Hadjipanayis CG, Norden AD, Shu HK, Wen PY, Olson JJ. Exciting new advances in neuro-oncology: the avenue to a cure for malignant glioma. CA Cancer J Clin. 2010;60(3):166-3. CrossRef
  2. Yu Y, Ramena G, Elble RC. The role of cancer stem cells in relapse of solid tumors. Front Biosci (Elite Ed). 2012;4:1528-1. CrossRef
  3. Hirst TC, Vesterinen HM, Sena ES, Egan KJ, Macleod MR, Whittle IR. Systematic review and meta-analysis of temozolomide in animal models of glioma: was clinical efficacy predicted? Br J Cancer. 2013;108(1):64-1. CrossRef
  4. Lam EW, Zwacka R, Engelhardt JF, Davidson BL, Domann FE Jr, Yan T, Oberley LW. Adenovirus-mediated manganese superoxide dismutase gene transfer to hamster cheek pouch carcinoma cells. Cancer Res. 1997;57(24):5550-.
  5. Grippo MC, Penteado PF, Carelli EF, Cruz-Hofling MA, Verinaud L. Establishment and partial characterization of a continuous human malignant glioma cell line: NG97. Cell Mol Neurobiol. 2001;21(4):421-. CrossRef
  6. Srivastava VK, Nalbantoglu J. Flow cytometric characterization of the DAOY medulloblastoma cell line for the cancer stem-like phenotype. Cytometry A. 2008;73(10):940-. CrossRef
  7. Sarkar S, Nuttall RK, Liu SH, Edwards DR, Yong VW. Tenascin-C stimulates glioma cell invasion through matrix metalloproteinase-12. Cancer Res. 2006;66(24):11771-0. CrossRef
  8. Challen GA, Little MH. A side order of stem cells: the SP phenotype. Stem Cells. 2006;24(1):3-2.
  9. Lendahl U, Zimmerman LB, McKay RD. CNS stem cells express a new class of intermediate filament protein. Cell. 1990;60(4):585-5. CrossRef
  10. Katsetos CD, Herman MM, Frankfurter A, Uffer S, Perentes E, Rubinstein LJ. Neuron-associated class III beta-tubulin isotype, microtubule-associated protein 2, and synaptophysin in human retinoblastomas in situ. Further immunohistochemical observations on the Flexner–Wintersteiner rosettes. Lab Invest. 1991;64(1):45-4.
  11. Boyer LA, Lee TI, Cole MF, Johnstone SE, Levine SS, Zucker JP, Guenther MG, Kumar RM, Murray HL, Jenner RG, et al. Core transcriptional regulatory circuitry in human embryonic stem cells. Cell. 2005;122(6):947-6. CrossRef
  12. Jiang J, Chan YS, Loh YH, Cai J, Tong GQ, Lim CA, Robson P, Zhong S, Ng HH. A core Klf circuitry regulates self-renewal of embryonic stem cells. Nat Cell Biol. 2008;10(3):353-0. CrossRef
  13. Pang R, Law WL, Chu AC, Poon JT, Lam CS, Chow AK, Ng L, Cheung LW, Lan XR, Lan HY, et al. A subpopulation of CD26?+?cancer stem cells with metastatic capacity in human colorectal cancer. Cell Stem Cell. 2010;6(6):603-5.
  14. Shi W, Wang H, Pan G, Geng Y, Guo Y, Pei D. Regulation of the pluripotency marker Rex-1 by Nanog and Sox2. J Biol Chem. 2006;281(33):23319-5. CrossRef
  15. Ben-Shushan E, Thompson JR, Gudas LJ, Bergman Y. Rex-1, a gene encoding a transcription factor expressed in the early embryo, is regulated via Oct-3/4 and Oct-6 binding to an octamer site and a novel protein, Rox-1, binding to an adjacent site. Mol Cell Biol. 1998;18(4):1866-8.
  16. Singh SK, Clarke ID, Terasaki M, Bonn VE, Hawkins C, Squire J, Dirks PB. Identification of a cancer stem cell in human brain tumors. Cancer Res. 2003;63(18):5821-.
  17. Hemmati HD, Nakano I, Lazareff JA, Masterman-Smith M, Geschwind DH, Bronner-Fraser M, Kornblum HI. Cancerous stem cells can arise from pediatric brain tumors. Proc Natl Acad Sci USA. 2003;100(25):15178-3. CrossRef
  18. Galli R, Binda E, Orfanelli U, Cipelletti B, Gritti A, De Vitis S, Fiocco R, Foroni C, Dimeco F, Vescovi A. Isolation and characterization of tumorigenic, stem-like neural precursors from human glioblastoma. Cancer Res. 2004;64(19):7011-1. CrossRef
  19. Tohyama T, Lee VM, Rorke LB, Marvin M, McKay RD, Trojanowski JQ. Nestin expression in embryonic human neuroepithelium and in human neuroepithelial tumor cells. Lab Invest. 1992;66(3):303-3.
  20. Duggal N, Iskander S, Hammond RR. MAP2 and nestin co-expression in dysembryoplastic neuroepithelial tumors. Clin Neuropathol. 2003;22(2):57-5.
  21. Fanarraga ML, Avila J, Zabala JC. Expression of unphosphorylated class III beta-tubulin isotype in neuroepithelial cells demonstrates neuroblast commitment and differentiation. Eur J Neurosci. 1999;11(2):517-7.
  22. Wan F, Herold-Mende C, Campos B, Centner FS, Dictus C, Becker N, Devens F, Mogler C, Felsberg J, Grabe N, et al. Association of stem cell-related markers and survival in astrocytic gliomas. Biomarkers. 2011;16(2):136-3. CrossRef
  23. Rushing EJ, Sandberg GD, Horkayne-Szakaly I. High-grade astrocytomas show increased nestin and Wilms’s tumor gene (WT1) protein expression. Int J Surg Pathol. 2010;18(4):255-. CrossRef
  24. Zhang M, Song T, Yang L, Chen R, Wu L, Yang Z, Fang J. Nestin and CD133: valuable stem cell-specific markers for determining clinical outcome of glioma patients. J Exp Clin Cancer Res. 2008;27:85. CrossRef
  25. Rutka JT, Ivanchuk S, Mondal S, Taylor M, Sakai K, Dirks P, Jun P, Jung S, Becker LE, Ackerley C. Co-expression of nestin and vimentin intermediate filaments in invasive human astrocytoma cells. Int J Dev Neurosci. 1999;17(5-):503-5. CrossRef
  26. Florenes VA, Holm R, Myklebost O, Lendahl U, Fodstad O. Expression of the neuroectodermal intermediate filament nestin in human melanomas. Cancer Res. 1994;54(2):354-.
  27. Holmberg J, He X, Peredo I, Orrego A, Hesselager G, Ericsson C, Hovatta O, Oba-Shinjo SM, Marie SK, Nister M, et al. Activation of neural and pluripotent stem cell signatures correlates with increased malignancy in human glioma. PLoS ONE. 2011;6(3):e18454. CrossRef
  28. Kajiwara K, Orita T, Nishizaki T, Kamiryo T, Nakayama H, Ito H. Glial fibrillary acidic protein (GFAP) expression and nucleolar organizer regions (NORs) in human gliomas. Brain Res. 1992;572(1-):314-. CrossRef
  29. Wang J, Sakariassen PO, Tsinkalovsky O, Immervoll H, Boe SO, Svendsen A, Prestegarden L, Rosland G, Thorsen F, Stuhr L, et al. CD133 negative glioma cells form tumors in nude rats and give rise to CD133 positive cells. Int J Cancer. 2008;122(4):761-. CrossRef
  
• 作者单位：Jiagui Qu (1) (2)
  Joshua D. Rizak (2) (3)
  Yaodong Fan (4)
  Xiaoxuan Guo (5)
  Jiejing Li (6)
  Tanzeel Huma (2) (7)
  Yuanye Ma (2)
  
  1. University of Science and Technology of China, Hefei, Anhui, 230026, People’s Republic of China
  2. State Key Laboratory of Brain and Cognitive Sciences, Kunming Institute of Zoology, Chinese Academy of Sciences, #32 Jiao Chang Dong Lu, Kunming, Yunnan, 650223, People’s Republic of China
  3. University of the Chinese Academy of Science, Beijing, 100101, People’s Republic of China
  4. Department of Neurosurgery, The Third Affiliated Hospital of Kunming Medical University (Yunnan Province Tumor Hospital), Kunming, Yunnan, 650118, People’s Republic of China
  5. Beijing Key Laboratory of Functional Food from Plant Resources, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, People’s Republic of China
  6. CAS-Max Planck Junior Scientist Group, State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650223, People’s Republic of China
  7. Reproductive Neuroendocrinology Laboratory, Department of Animal Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, 45320, Pakistan
  
• ISSN：1749-0774

文摘

This paper outlines the establishment of a new and stable cell line, designated GBM-HSF, from a malignant glioblastoma multiforme (GBM) removed from a 65-year-old Chinese woman. This cell line has been grown for 1 year without disruption and has been passaged over 50 times. The cells were adherently cultured in RPMI-1640 media with 10?% fetal bovine serum supplementation. Cells displayed spindle and polygonal morphology, and displayed multi-layered growth without evidence of contact inhibition. The cell line had a high growth rate with a doubling time of 51?h. The cells were able to grow without adhering to the culture plates, and 4.5?% of the total cells formed colonies in soft agar. The cell line has also been found to form tumors in nude mice and to be of a highly invasive nature. The cells were also partially characterized with RT-PCR. The RT-PCR revealed that Nestin, β-tubulin III, Map2, Klf4, Oct4, Sox2, Nanog, and CD26 were positively transcribed, whereas GFAP, Rex1, and CD133 were negatively transcribed in this cell line. These results suggest that the GBM-HSF cell line will provide a good model to study the properties of cancer stem cells and metastasis. It will also facilitate more detailed molecular and cellular studies of GBM cell division and pathology.