Cordycepin prevented IL-β-induced expression of inflammatory mediators in human osteoarthritis chondrocytes

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• 作者：Xiaozhou Ying (1)
  Lei Peng (2)
  Hua Chen (1)
  Yue Shen (1)
  Kehe Yu (1)
  Shaowen Cheng (2)
  
• 关键词：Cordycepin ; Interleukin ; 1β (IL ; 1β) ; Nitric oxide ; Osteoarthritis
• 刊名：International Orthopaedics
• 出版年：2014
• 出版时间：July 2014
• 年：2014
• 卷：38
• 期：7
• 页码：1519-1526
• 全文大小：757 KB
• 参考文献：1. Flugge LA, Miller-Deist LA, Petillo PA (1999) Towards a molecular understanding of arthritis. Chem Biol 6(6):R157-66. doi:10.1016/s1074-5521(99)80043-x CrossRef
  2. Goldring MB, Otero M, Tsuchimochi K, Ijiri K, Li Y (2008) Defining the roles of inflammatory and anabolic cytokines in cartilage metabolism. Ann Rheum Dis 67(Suppl 3):iii75-2. doi:10.1136/ard.2008.098764 CrossRef
  3. Loeser RF (2006) Molecular mechanisms of cartilage destruction: mechanics, inflammatory mediators, and aging collide. Arthritis Rheum 54(5):1357-360. doi:10.1002/art.21813 CrossRef
  4. Pelletier JP, Martel-Pelletier J, Abramson SB (2001) Osteoarthritis, an inflammatory disease: potential implication for the selection of new therapeutic targets. Arthritis Rheum 44(6):1237-247. doi:10.1002/1529-0131(200106)44:6<1237::aid-art214>3.0.co;2-f CrossRef
  5. Kobayashi M, Squires GR, Mousa A, Tanzer M, Zukor DJ, Antoniou J, Feige U, Poole AR (2005) Role of interleukin-1 and tumor necrosis factor alpha in matrix degradation of human osteoarthritic cartilage. Arthritis Rheum 52(1):128-35. doi:10.1002/art.20776 CrossRef
  6. Goldring SR, Goldring MB (2004) The role of cytokines in cartilage matrix degeneration in osteoarthritis. Clin Orthop Relat Res 427 Suppl:S27-6 CrossRef
  7. Pelletier JP, Caron JP, Evans C, Robbins PD, Georgescu HI, Jovanovic D, Fernandes JC, Martel-Pelletier J (1997) In vivo suppression of early experimental osteoarthritis by interleukin-1 receptor antagonist using gene therapy. Arthritis Rheum 40(6):1012-019. doi:10.1002/1529-0131(199706)40:6<1012::AID-ART3>3.0.CO;2-# CrossRef
  8. Han ES, Oh JY, Park HJ (2011) Cordyceps militaris extract suppresses dextran sodium sulfate-induced acute colitis in mice and production of inflammatory mediators from macrophages and mast cells. J Ethnopharmacol 134(3):703-10. doi:10.1016/j.jep.2011.01.022 CrossRef
  9. Hubbell HR, Pequignot EC, Willis DH, Lee C, Suhadolnik RJ (1985) Differential antiproliferative actions of 2-5-oligo A trimer core and its cordycepin analogue on human tumor cells. Int J Cancer 36(3):389-94
  10. Won SY, Park EH (2005) Anti-inflammatory and related pharmacological activities of cultured mycelia and fruiting bodies of Cordyceps militaris. J Ethnopharmacol 96(3):555-61. doi:10.1016/j.jep.2004.10.009 CrossRef
  11. Lee HH, Jeong JW, Lee JH, Kim GY, Cheong J, Jeong YK, Yoo YH, Choi YH (2013) Cordycepin increases sensitivity of Hep3B human hepatocellular carcinoma cells to TRAIL-mediated apoptosis by inactivating the JNK signaling pathway. Oncol Rep. doi:10.3892/or.2013.2589
  12. Sjoholm A, Nystrom T (2005) Endothelial inflammation in insulin resistance. Lancet 365(9459):610-12. doi:10.1016/s0140-6736(05)17912-4 CrossRef
  13. Yoo HS, Shin JW, Cho JH, Son CG, Lee YW, Park SY, Cho CK (2004) Effects of Cordyceps militaris extract on angiogenesis and tumor growth. Acta Pharmacol Sin 25(5):657-65
  14. Kan WC, Wang HY, Chien CC, Li SL, Chen YC, Chang LH, Cheng CH, Tsai WC, Hwang JC, Su SB, Huang LH, Chuu JJ (2012) Effects of Extract from Solid-State Fermented Cordyceps sinensis on Type 2 Diabetes Mellitus. Evid Based Complement Alternat Med 2012:743107. doi:10.1155/2012/743107 CrossRef
  15. Li L, He D, Yang J, Wang X (2011) Cordycepin inhibits renal interstitial myofibroblast activation probably by inducing hepatocyte growth factor expression. J Pharmacol Sci 117(4):286-94 CrossRef
  16. Noh EM, Kim JS, Hur H, Park BH, Song EK, Han MK, Kwon KB, Yoo WH, Shim IK, Lee SJ, Youn HJ, Lee YR (2009) Cordycepin inhibits IL-1beta-induced MMP-1 and MMP-3 expression in rheumatoid arthritis synovial fibroblasts. Rheumatology (Oxford) 48(1):45-8. doi:10.1093/rheumatology/ken417 CrossRef
  17. Shin S, Lee S, Kwon J, Moon S, Lee CK, Cho K, Ha NJ, Kim K (2009) Cordycepin Suppresses Expression of Diabetes Regulating Genes by Inhibition of Lipopolysaccharide-induced Inflammation in Macrophages. Immune Netw 9(3):98-05. doi:10.4110/in.2009.9.3.98 CrossRef
  18. Baik JS, Kwon HY, Kim KS, Jeong YK, Cho YS, Lee YC (2012) Cordycepin induces apoptosis in human neuroblastoma SK-N-BE(2)-C and melanoma SK-MEL-2 cells. Indian J Biochem Biophys 49(2):86-1
  19. Jeong MH, Seo MJ, Park JU, Kang BW, Kim KS, Lee JY, Kim GY, Kim JI, Choi YH, Kim KH, Jeong YK (2012) Effect of cordycepin purified from Cordyceps militaris on Th1 and Th2 cytokines in mouse splenocytes. J Microbiol Biotechnol 22(8):1161-164 CrossRef
  20. Lee HJ, Burger P, Vogel M, Friese K, Bruning A (2012) The nucleoside antagonist cordycepin causes DNA double strand breaks in breast cancer cells. Investig New Drugs 30(5):1917-925. doi:10.1007/s10637-012-9859-x CrossRef
  21. Liu Q, Hong IP, Ahn MJ, Yoo HS, Han SB, Hwang BY, Lee MK (2011) Anti-adipogenic activity of Cordyceps militaris in 3?T3-L1 cells. Nat Prod Commun 6(12):1839-841
  22. Wu CW, Morrell MR, Heinze E, Concoff AL, Wollaston SJ, Arnold EL, Singh R, Charles C, Skovrun ML, FitzGerald JD, Moreland LW, Kalunian KC (2005) Validation of American College of Rheumatology classification criteria for knee osteoarthritis using arthroscopically defined cartilage damage scores. Semin Arthritis Rheum 35(3):197-01. doi:10.1016/j.semarthrit.2005.06.002 CrossRef
  23. Ying X, Cheng S, Shen Y, Cheng X, An Rompis F, Wang W, Lin Z, Chen Q, Zhang W, Kou D, Peng L, Tian XQ, Lu CZ (2012) Nicotine promotes proliferation and collagen synthesis of chondrocytes isolated from normal human and osteoarthritis patients. Mol Cell Biochem 359(1-):263-69. doi:10.1007/s11010-011-1020-1 CrossRef
  24. Ying X, Chen X, Cheng S, Shen Y, Peng L, Xu HZ (2013) Piperine inhibits IL-beta induced expression of inflammatory mediators in human osteoarthritis chondrocyte. Int Immunopharmacol. doi:10.1016/j.intimp.2013.06.025
  25. Akhtar N, Miller MJ, Haqqi TM (2011) Effect of a Herbal-Leucine mix on the IL-1beta-induced cartilage degradation and inflammatory gene expression in human chondrocytes. BMC Complement Altern Med 11:66. doi:10.1186/1472-6882-11-66 CrossRef
  26. Kim KS, da Oh H, Choi HM, Bang JS, Ryu CJ, Kim JH, Yoo MC, Yang HI (2009) Pyrrolidine dithiocarbamate, a NF-kappaB inhibitor, upregulates MMP-1 and MMP-13 in IL-1beta-stimulated rheumatoid arthritis fibroblast-like synoviocytes. Eur J Pharmacol 613(1-):167-75. doi:10.1016/j.ejphar.2009.04.026 CrossRef
  27. Wei XQ, Charles IG, Smith A, Ure J, Feng GJ, Huang FP, Xu D, Muller W, Moncada S, Liew FY (1995) Altered immune responses in mice lacking inducible nitric oxide synthase. Nature 375(6530):408-11. doi:10.1038/375408a0 CrossRef
  28. Heard BJ, Martin L, Rattner JB, Frank CB, Hart DA, Krawetz R (2012) Matrix metalloproteinase protein expression profiles cannot distinguish between normal and early osteoarthritic synovial fluid. BMC Musculoskelet Disord 13:126. doi:10.1186/1471-2474-13-126 CrossRef
  29. Mitchell PG, Magna HA, Reeves LM, Lopresti-Morrow LL, Yocum SA, Rosner PJ, Geoghegan KF, Hambor JE (1996) Cloning, expression, and type II collagenolytic activity of matrix metalloproteinase-13 from human osteoarthritic cartilage. J Clin Invest 97(3):761-68. doi:10.1172/jci118475 CrossRef
  30. Little CB, Barai A, Burkhardt D, Smith SM, Fosang AJ, Werb Z, Shah M, Thompson EW (2009) Matrix metalloproteinase 13-deficient mice are resistant to osteoarthritic cartilage erosion but not chondrocyte hypertrophy or osteophyte development. Arthritis Rheum 60(12):3723-733. doi:10.1002/art.25002 CrossRef
  31. Flannery CR, Little CB, Hughes CE, Curtis CL, Caterson B, Jones SA (2000) IL-6 and its soluble receptor augment aggrecanase-mediated proteoglycan catabolism in articular cartilage. Matrix Biol 19(6):549-53 CrossRef
  32. Tak PP, Firestein GS (2001) NF-kappaB: a key role in inflammatory diseases. J Clin Invest 107(1):7-1. doi:10.1172/jci11830 CrossRef
  33. Gilston V, Jones HW, Soo CC, Coumbe A, Blades S, Kaltschmidt C, Baeuerle PA, Morris CJ, Blake DR, Winyard PG (1997) NF-kappa B activation in human knee-joint synovial tissue during the early stage of joint inflammation. Biochem Soc Trans 25(3):518S
  34. Miagkov AV, Kovalenko DV, Brown CE, Didsbury JR, Cogswell JP, Stimpson SA, Baldwin AS, Makarov SS (1998) NF-kappaB activation provides the potential link between inflammation and hyperplasia in the arthritic joint. Proc Natl Acad Sci U S A 95(23):13859-3864 CrossRef
  35. Lu HT, Liang YC, Sheu MT, Ho HO, Lin YT, Hsieh MS, Chen CH (2008) Disease-modifying effects of glucosamine HCl involving regulation of metalloproteinases and chemokines activated by interleukin-1beta in human primary synovial fibroblasts. J Cell Biochem 104(1):38-0. doi:10.1002/jcb.21597 CrossRef
  36. Ren Z, Cui J, Huo Z, Xue J, Cui H, Luo B, Jiang L, Yang R (2012) Cordycepin suppresses TNF-alpha-induced NF-kappaB activation by reducing p65 transcriptional activity, inhibiting IkappaBalpha phosphorylation, and blocking IKKgamma ubiquitination. Int Immunopharmacol 14(4):698-03. doi:10.1016/j.intimp.2012.10.008 CrossRef
  
• 作者单位：Xiaozhou Ying (1)
  Lei Peng (2)
  Hua Chen (1)
  Yue Shen (1)
  Kehe Yu (1)
  Shaowen Cheng (2)
  
  1. Department of Orthopaedic Surgery, The Second Affiliated Hospital of Wenzhou Medical College, 109 Xue yuan xi Road, Wenzhou, 325000, China
  2. Trauma Center of the Affiliated Hospital of Hainan Medical College, Haikou, 570100, China
  
• ISSN：1432-5195

文摘

Purpose Cordycepin, a nucleoside derivative isolated from Cordyceps, has been reported to exert anti-inflammatory, antitumor, antidiabetic and renoprotective effects. Osteoarthritis (OA) is a degenerative joint disease with an inflammatory component that drives the degradation of cartilage extracellular matrix. This study aimed to assess the effects of cordycepin on human OA chondrocytes. Methods In this study, human OA chondrocytes were pretreated with cordycepin at 10, 50 or 100?μM and subsequently stimulated with interleukin-1β (IL-1β) (5?ng/ml) for 24?h. Production of prostaglandin E2 (PGE2) and nitric oxide (NO) were evaluated by the Griess reaction and an enzyme-linked immunosorbent assay (ELISA). Gene expression of matrix metalloproteinase (MMP)-13, IL-6, inducible nitric oxide synthase (iNOS) and cyclo-oxygenase (COX-2) was measured by real-time polymerase chain reaction (PCR). MMP-13 and IL-6 proteins in culture medium were determined using cytokine-specific ELISA. Western immunoblotting was used to analyse the iNOS and COX-2 protein production in culture medium. Nuclear factor kappa-B (NF-κB) activity regulation was explored using Western immunoblotting. Results Pretreatment with cordycepin significantly inhibited the production of PGE2 and NO induced by IL-1β. Cordycepin also significantly decreased the IL-1β-stimulated gene expression and production of MMP-13, IL-6, iNOS and COX-2 in OA chondrocytes. Pretreatment with cordycepin attenuated IL-1β-induced activation of NF-κB by suppressing degradation of its inhibitory protein nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (IκB-α) in the cytoplasm. Conclusions We show for the first time the anti-inflammatory activity of cordycepin in human OA chondrocytes. Thus, with this unique profile of actions, cordycepin may prove to be a potentially attractive and new therapeutic/preventive agent for OA.