The second transcribed spacer rDNA sequence: an effective genetic marker for inter-species phylogenetic analysis of trematodes in the order Strigeata

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• 作者：G. H. Zhao (12)
  J. Li (145)
  X. H. Mo (1)
  X. Y. Li (13)
  R. Q. Lin (5)
  F. C. Zou (6)
  Y. B. Weng (5)
  H. Q. Song (15)
  X. Q. Zhu (16) xingquanzhu1@hotmail.com
• 刊名：Parasitology Research
• 出版年：2012
• 出版时间：October 2012
• 年：2012
• 卷：111
• 期：4
• 页码：1467-1472
• 全文大小：235.4 KB
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• 作者单位：1. State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou, Gansu Province 730046, People鈥檚 Republic of China2. College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi Province 712100, People鈥檚 Republic of China3. College of Agronomy, Northwest A & F University, Yangling, Shaanxi Province 712100, People鈥檚 Republic of China4. Institute of Veterinary Medicine, Guangdong Academy of Agricultural Sciences, Guangzhou, Guangdong Province 510642, People鈥檚 Republic of China5. College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province 510642, People鈥檚 Republic of China6. College of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan Province 650201, People鈥檚 Republic of China
• ISSN：1432-1955

文摘

In the present study, the second nuclear internal transcribed spacer (ITS-2) rDNA of Schistosoma japonicum isolates in mainland China was amplified, sequenced, and assessed for inferring the intra- and inter-species phylogenetic relationships of trematodes in the order Strigeata. The fragment containing ITS-2 rDNA was obtained from 24 S. japonicum isolates from eight epidemic provinces in mainland China. The length polymorphisms were observed among these ITS-2 rDNA sequences, ranging from 343 to 346 bp, and the intra- and inter-population variations in ITS-2 sequence were 0.0–2.1 % among S. japonicum isolates in China. Phylogenetic analyses using the maximum parsimony and maximum likelihood methods revealed that the ITS-2 rDNA sequence is not a suitable marker for studying inter- and intra-population variation in S. japonicum. However, phylogenetic analysis of trematodes in the order Strigeata indicated that the ITS-2 rDNA sequence provides an effective molecular marker for studying inter-species phylogenetic relationships among trematodes in this order.